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Metabolic Signature of Pluripotent Stem Cells

OBJECTIVE: Pluripotent stem cells (PSCs), with the capacity to self-renew and differentiate into all other cell types, are of benefit in regenerative medicine applications. Tightly controlled gene expression networks and epigenetic factors regulate these properties. In this study, we aim to evaluate...

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Autores principales: Taleahmad, Sara, Hassani, Seyedeh Nafiseh, Salekdeh, Ghasem Hosseini, Baharvand, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004998/
https://www.ncbi.nlm.nih.gov/pubmed/29845793
http://dx.doi.org/10.22074/cellj.2018.5514
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author Taleahmad, Sara
Hassani, Seyedeh Nafiseh
Salekdeh, Ghasem Hosseini
Baharvand, Hossein
author_facet Taleahmad, Sara
Hassani, Seyedeh Nafiseh
Salekdeh, Ghasem Hosseini
Baharvand, Hossein
author_sort Taleahmad, Sara
collection PubMed
description OBJECTIVE: Pluripotent stem cells (PSCs), with the capacity to self-renew and differentiate into all other cell types, are of benefit in regenerative medicine applications. Tightly controlled gene expression networks and epigenetic factors regulate these properties. In this study, we aim to evaluate the metabolic signature of pluripotency under 2i and R2i culture conditions versus serum condition. MATERIALS AND METHODS: In this experimental study, we investigated bioinformatics analysis of the shotgun proteomics data for cells grown under 2i, R2i, and serum culture conditions. The findings were validated by cell cycle analysis and gene expressions of the cells with flow cytometry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), respectively. RESULTS: Expressions of 163 proteins increased in 2i-grown cells and 181 proteins increased in R2i-grown cells versus serum, which were mostly involved in glycolysis signaling pathway, oxidation-reduction, metabolic processes, amino acid and lipid metabolism. Flow cytometry analysis showed significant accumulation of cells in S phase for 2i (70%) and R2i (61%) grown cells. CONCLUSION: This study showed that under 2i and R2i conditions, glycolysis was highlighted for energy production and used to maintain high levels of glycolytic intermediates to support cell proliferation. Cells grown under 2i and R2i conditions showed rapid cell cycling in comparison with the cells grown under serum conditions.
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spelling pubmed-60049982018-09-01 Metabolic Signature of Pluripotent Stem Cells Taleahmad, Sara Hassani, Seyedeh Nafiseh Salekdeh, Ghasem Hosseini Baharvand, Hossein Cell J Original Article OBJECTIVE: Pluripotent stem cells (PSCs), with the capacity to self-renew and differentiate into all other cell types, are of benefit in regenerative medicine applications. Tightly controlled gene expression networks and epigenetic factors regulate these properties. In this study, we aim to evaluate the metabolic signature of pluripotency under 2i and R2i culture conditions versus serum condition. MATERIALS AND METHODS: In this experimental study, we investigated bioinformatics analysis of the shotgun proteomics data for cells grown under 2i, R2i, and serum culture conditions. The findings were validated by cell cycle analysis and gene expressions of the cells with flow cytometry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), respectively. RESULTS: Expressions of 163 proteins increased in 2i-grown cells and 181 proteins increased in R2i-grown cells versus serum, which were mostly involved in glycolysis signaling pathway, oxidation-reduction, metabolic processes, amino acid and lipid metabolism. Flow cytometry analysis showed significant accumulation of cells in S phase for 2i (70%) and R2i (61%) grown cells. CONCLUSION: This study showed that under 2i and R2i conditions, glycolysis was highlighted for energy production and used to maintain high levels of glycolytic intermediates to support cell proliferation. Cells grown under 2i and R2i conditions showed rapid cell cycling in comparison with the cells grown under serum conditions. Royan Institute 2018 2018-05-28 /pmc/articles/PMC6004998/ /pubmed/29845793 http://dx.doi.org/10.22074/cellj.2018.5514 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Taleahmad, Sara
Hassani, Seyedeh Nafiseh
Salekdeh, Ghasem Hosseini
Baharvand, Hossein
Metabolic Signature of Pluripotent Stem Cells
title Metabolic Signature of Pluripotent Stem Cells
title_full Metabolic Signature of Pluripotent Stem Cells
title_fullStr Metabolic Signature of Pluripotent Stem Cells
title_full_unstemmed Metabolic Signature of Pluripotent Stem Cells
title_short Metabolic Signature of Pluripotent Stem Cells
title_sort metabolic signature of pluripotent stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004998/
https://www.ncbi.nlm.nih.gov/pubmed/29845793
http://dx.doi.org/10.22074/cellj.2018.5514
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