Cargando…
The Effects of Sodium Selenite on Mitochondrial DNA Copy Number and Reactive Oxygen Species Levels of In Vitro Matured Mouse Oocytes
OBJECTIVE: The aim of present study is to determine the effects of supplementation of oocyte maturation medium with sodium selenite (SS) on oocyte mitochondrial DNA (mtDNA) copy number and reactive oxygen species (ROS) levels. MATERIALS AND METHODS: In this experimental study germinal vesicle (GV),...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004999/ https://www.ncbi.nlm.nih.gov/pubmed/29845794 http://dx.doi.org/10.22074/cellj.2018.5430 |
_version_ | 1783332627664601088 |
---|---|
author | Ghorbanmehr, Nassim Salehnia, Mojdeh Amooshahi, Mahboobeh |
author_facet | Ghorbanmehr, Nassim Salehnia, Mojdeh Amooshahi, Mahboobeh |
author_sort | Ghorbanmehr, Nassim |
collection | PubMed |
description | OBJECTIVE: The aim of present study is to determine the effects of supplementation of oocyte maturation medium with sodium selenite (SS) on oocyte mitochondrial DNA (mtDNA) copy number and reactive oxygen species (ROS) levels. MATERIALS AND METHODS: In this experimental study germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) stage oocytes were recovered from 6-8 week old female mice after superovulation. Some of the GV oocytes were cultured and matured in the presence and absence of SS. Then in vivo and in vitro matured (IVM) oocytes were subjected to mitochondria staining by MitoTracker green, ROS analysis, and mtDNA copy number determination using absolute real-time polymerase chain reaction (PCR). RESULTS: The maturation rate of GV oocytes to the MII stage significantly increased in the SS supplemented group (79.25%) compared to the control group (72.46%, P<0.05). The intensity of mitochondrial staining was not different among the studied groups, whereas the mitochondria distribution in the cytoplasm of the IVM oocytes showed some aggregation pattern. The in vivo obtained MII oocytes had lower ROS levels and higher mtDNA copy numbers than IVM-MII oocytes (P<0.05). The SS supplemented group had significantly lower ROS levels and higher mtDNA copy numbers than the non-treated group (P<0.05). CONCLUSION: SS increased oocyte mtDNA copy number by decreasing oxidative stress. SS had an association with better oocyte developmental competence. |
format | Online Article Text |
id | pubmed-6004999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-60049992018-09-01 The Effects of Sodium Selenite on Mitochondrial DNA Copy Number and Reactive Oxygen Species Levels of In Vitro Matured Mouse Oocytes Ghorbanmehr, Nassim Salehnia, Mojdeh Amooshahi, Mahboobeh Cell J Original Article OBJECTIVE: The aim of present study is to determine the effects of supplementation of oocyte maturation medium with sodium selenite (SS) on oocyte mitochondrial DNA (mtDNA) copy number and reactive oxygen species (ROS) levels. MATERIALS AND METHODS: In this experimental study germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) stage oocytes were recovered from 6-8 week old female mice after superovulation. Some of the GV oocytes were cultured and matured in the presence and absence of SS. Then in vivo and in vitro matured (IVM) oocytes were subjected to mitochondria staining by MitoTracker green, ROS analysis, and mtDNA copy number determination using absolute real-time polymerase chain reaction (PCR). RESULTS: The maturation rate of GV oocytes to the MII stage significantly increased in the SS supplemented group (79.25%) compared to the control group (72.46%, P<0.05). The intensity of mitochondrial staining was not different among the studied groups, whereas the mitochondria distribution in the cytoplasm of the IVM oocytes showed some aggregation pattern. The in vivo obtained MII oocytes had lower ROS levels and higher mtDNA copy numbers than IVM-MII oocytes (P<0.05). The SS supplemented group had significantly lower ROS levels and higher mtDNA copy numbers than the non-treated group (P<0.05). CONCLUSION: SS increased oocyte mtDNA copy number by decreasing oxidative stress. SS had an association with better oocyte developmental competence. Royan Institute 2018 2018-05-28 /pmc/articles/PMC6004999/ /pubmed/29845794 http://dx.doi.org/10.22074/cellj.2018.5430 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ghorbanmehr, Nassim Salehnia, Mojdeh Amooshahi, Mahboobeh The Effects of Sodium Selenite on Mitochondrial DNA Copy Number and Reactive Oxygen Species Levels of In Vitro Matured Mouse Oocytes |
title | The Effects of Sodium Selenite on Mitochondrial DNA Copy
Number and Reactive Oxygen Species Levels of In Vitro
Matured Mouse Oocytes |
title_full | The Effects of Sodium Selenite on Mitochondrial DNA Copy
Number and Reactive Oxygen Species Levels of In Vitro
Matured Mouse Oocytes |
title_fullStr | The Effects of Sodium Selenite on Mitochondrial DNA Copy
Number and Reactive Oxygen Species Levels of In Vitro
Matured Mouse Oocytes |
title_full_unstemmed | The Effects of Sodium Selenite on Mitochondrial DNA Copy
Number and Reactive Oxygen Species Levels of In Vitro
Matured Mouse Oocytes |
title_short | The Effects of Sodium Selenite on Mitochondrial DNA Copy
Number and Reactive Oxygen Species Levels of In Vitro
Matured Mouse Oocytes |
title_sort | effects of sodium selenite on mitochondrial dna copy
number and reactive oxygen species levels of in vitro
matured mouse oocytes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004999/ https://www.ncbi.nlm.nih.gov/pubmed/29845794 http://dx.doi.org/10.22074/cellj.2018.5430 |
work_keys_str_mv | AT ghorbanmehrnassim theeffectsofsodiumseleniteonmitochondrialdnacopynumberandreactiveoxygenspecieslevelsofinvitromaturedmouseoocytes AT salehniamojdeh theeffectsofsodiumseleniteonmitochondrialdnacopynumberandreactiveoxygenspecieslevelsofinvitromaturedmouseoocytes AT amooshahimahboobeh theeffectsofsodiumseleniteonmitochondrialdnacopynumberandreactiveoxygenspecieslevelsofinvitromaturedmouseoocytes AT ghorbanmehrnassim effectsofsodiumseleniteonmitochondrialdnacopynumberandreactiveoxygenspecieslevelsofinvitromaturedmouseoocytes AT salehniamojdeh effectsofsodiumseleniteonmitochondrialdnacopynumberandreactiveoxygenspecieslevelsofinvitromaturedmouseoocytes AT amooshahimahboobeh effectsofsodiumseleniteonmitochondrialdnacopynumberandreactiveoxygenspecieslevelsofinvitromaturedmouseoocytes |