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Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis
During organismal development, cells undergo complex changes in shape whose causal relationship to individual morphogenetic processes remains unclear. The modular nature of such processes suggests that it should be possible to isolate individual modules, determine the minimum set of requirements suf...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006163/ https://www.ncbi.nlm.nih.gov/pubmed/29915285 http://dx.doi.org/10.1038/s41467-018-04754-z |
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author | Izquierdo, Emiliano Quinkler, Theresa De Renzis, Stefano |
author_facet | Izquierdo, Emiliano Quinkler, Theresa De Renzis, Stefano |
author_sort | Izquierdo, Emiliano |
collection | PubMed |
description | During organismal development, cells undergo complex changes in shape whose causal relationship to individual morphogenetic processes remains unclear. The modular nature of such processes suggests that it should be possible to isolate individual modules, determine the minimum set of requirements sufficient to drive tissue remodeling, and re-construct morphogenesis. Here we use optogenetics to reconstitute epithelial folding in embryonic Drosophila tissues that otherwise would not undergo invagination. We show that precise spatial and temporal activation of Rho signaling is sufficient to trigger apical constriction and tissue folding. Induced furrows can occur at any position along the dorsal–ventral or anterior–posterior embryo axis in response to the spatial pattern and level of optogenetic activation. Thus, epithelial folding is a direct function of the spatio-temporal organization and strength of Rho signaling that on its own is sufficient to drive tissue internalization independently of any pre-determined condition or differentiation program associated with endogenous invagination processes. |
format | Online Article Text |
id | pubmed-6006163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60061632018-06-20 Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis Izquierdo, Emiliano Quinkler, Theresa De Renzis, Stefano Nat Commun Article During organismal development, cells undergo complex changes in shape whose causal relationship to individual morphogenetic processes remains unclear. The modular nature of such processes suggests that it should be possible to isolate individual modules, determine the minimum set of requirements sufficient to drive tissue remodeling, and re-construct morphogenesis. Here we use optogenetics to reconstitute epithelial folding in embryonic Drosophila tissues that otherwise would not undergo invagination. We show that precise spatial and temporal activation of Rho signaling is sufficient to trigger apical constriction and tissue folding. Induced furrows can occur at any position along the dorsal–ventral or anterior–posterior embryo axis in response to the spatial pattern and level of optogenetic activation. Thus, epithelial folding is a direct function of the spatio-temporal organization and strength of Rho signaling that on its own is sufficient to drive tissue internalization independently of any pre-determined condition or differentiation program associated with endogenous invagination processes. Nature Publishing Group UK 2018-06-18 /pmc/articles/PMC6006163/ /pubmed/29915285 http://dx.doi.org/10.1038/s41467-018-04754-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Izquierdo, Emiliano Quinkler, Theresa De Renzis, Stefano Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis |
title | Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis |
title_full | Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis |
title_fullStr | Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis |
title_full_unstemmed | Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis |
title_short | Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis |
title_sort | guided morphogenesis through optogenetic activation of rho signalling during early drosophila embryogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006163/ https://www.ncbi.nlm.nih.gov/pubmed/29915285 http://dx.doi.org/10.1038/s41467-018-04754-z |
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