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Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging
With the capability of presenting endogenous tissue contrast or exogenous contrast agents in deep biological samples at high spatial resolution, photoacoustic (PA) imaging has shown significant potential for many preclinical and clinical applications. However, due to strong background signals from v...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006254/ https://www.ncbi.nlm.nih.gov/pubmed/29915177 http://dx.doi.org/10.1038/s41598-018-27578-9 |
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author | Tan, Joel W. Y. Lee, Chang H. Kopelman, Raoul Wang, Xueding |
author_facet | Tan, Joel W. Y. Lee, Chang H. Kopelman, Raoul Wang, Xueding |
author_sort | Tan, Joel W. Y. |
collection | PubMed |
description | With the capability of presenting endogenous tissue contrast or exogenous contrast agents in deep biological samples at high spatial resolution, photoacoustic (PA) imaging has shown significant potential for many preclinical and clinical applications. However, due to strong background signals from various intrinsic chromophores in biological tissue, such as hemoglobin, achieving highly sensitive PA imaging of targeting probes labeled by contrast agents has remained a challenge. In this study, we introduce a novel technique called transient triplet differential (TTD) imaging which allows for substantial reduction of tissue background signals. TTD imaging detects directly the triplet state absorption, which is a special characteristic of phosphorescence capable dyes not normally present among intrinsic chromophores of biological tissue. Thus, these triplet state absorption PA images can facilitate “true” background free molecular imaging. We prepared a known phosphorescent dye probe, methylene blue conjugated polyacrylamide nanoparticles, with peak absorption at 660 nm and peak lowest triplet state absorption at 840 nm. We find, through studies on phantoms and on an in vivo tumor model, that TTD imaging can generate a superior contrast-to-noise ratio, compared to other image enhancement techniques, through the removal of noise generated by strongly absorbing intrinsic chromophores, regardless of their identity. |
format | Online Article Text |
id | pubmed-6006254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60062542018-06-26 Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging Tan, Joel W. Y. Lee, Chang H. Kopelman, Raoul Wang, Xueding Sci Rep Article With the capability of presenting endogenous tissue contrast or exogenous contrast agents in deep biological samples at high spatial resolution, photoacoustic (PA) imaging has shown significant potential for many preclinical and clinical applications. However, due to strong background signals from various intrinsic chromophores in biological tissue, such as hemoglobin, achieving highly sensitive PA imaging of targeting probes labeled by contrast agents has remained a challenge. In this study, we introduce a novel technique called transient triplet differential (TTD) imaging which allows for substantial reduction of tissue background signals. TTD imaging detects directly the triplet state absorption, which is a special characteristic of phosphorescence capable dyes not normally present among intrinsic chromophores of biological tissue. Thus, these triplet state absorption PA images can facilitate “true” background free molecular imaging. We prepared a known phosphorescent dye probe, methylene blue conjugated polyacrylamide nanoparticles, with peak absorption at 660 nm and peak lowest triplet state absorption at 840 nm. We find, through studies on phantoms and on an in vivo tumor model, that TTD imaging can generate a superior contrast-to-noise ratio, compared to other image enhancement techniques, through the removal of noise generated by strongly absorbing intrinsic chromophores, regardless of their identity. Nature Publishing Group UK 2018-06-18 /pmc/articles/PMC6006254/ /pubmed/29915177 http://dx.doi.org/10.1038/s41598-018-27578-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tan, Joel W. Y. Lee, Chang H. Kopelman, Raoul Wang, Xueding Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging |
title | Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging |
title_full | Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging |
title_fullStr | Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging |
title_full_unstemmed | Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging |
title_short | Transient Triplet Differential (TTD) Method for Background Free Photoacoustic Imaging |
title_sort | transient triplet differential (ttd) method for background free photoacoustic imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006254/ https://www.ncbi.nlm.nih.gov/pubmed/29915177 http://dx.doi.org/10.1038/s41598-018-27578-9 |
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