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Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay
A new fluorometric assay is presented for the ultrasensitive quantification of total protein carbonyls, and is based on their specific reaction with rhodamine B hydrazide (RBH), and the production of a protein carbonyl-RBH hydrazone the fluorescence of which (at ex/em 560/585 nm) is greatly enhanced...
Autores principales: | Georgiou, Christos D., Zisimopoulos, Dimitrios, Argyropoulou, Vasiliki, Kalaitzopoulou, Electra, Ioannou, Panayiotis V., Salachas, George, Grune, Tilman |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006725/ https://www.ncbi.nlm.nih.gov/pubmed/29727801 http://dx.doi.org/10.1016/j.redox.2018.04.017 |
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