Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections

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Improved diagnostics are needed for children with musculoskeletal infections (MSKIs). We assessed the performance of target-enriched multiplex polymerase chain reaction (TEM-PCR) in children with MSKI. TEM-PCR was concordant with culture in pathogen identification and antibiotic susceptibility testi...

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Autores principales: Wood, James B, Sesler, Cheryl, Stalons, Donald, Grigorenko, Elena, Schoenecker, Jonathan G, Creech, C Buddy, Thomsen, Isaac P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007387/
https://www.ncbi.nlm.nih.gov/pubmed/29977969
http://dx.doi.org/10.1093/ofid/ofy119
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author Wood, James B
Sesler, Cheryl
Stalons, Donald
Grigorenko, Elena
Schoenecker, Jonathan G
Creech, C Buddy
Thomsen, Isaac P
author_facet Wood, James B
Sesler, Cheryl
Stalons, Donald
Grigorenko, Elena
Schoenecker, Jonathan G
Creech, C Buddy
Thomsen, Isaac P
author_sort Wood, James B
collection PubMed
description Improved diagnostics are needed for children with musculoskeletal infections (MSKIs). We assessed the performance of target-enriched multiplex polymerase chain reaction (TEM-PCR) in children with MSKI. TEM-PCR was concordant with culture in pathogen identification and antibiotic susceptibility testing, while increasing the overall yield of pathogen detection. This technology has the potential to inform judicious antimicrobial use early in the disease course.
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spelling pubmed-60073872018-07-05 Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections Wood, James B Sesler, Cheryl Stalons, Donald Grigorenko, Elena Schoenecker, Jonathan G Creech, C Buddy Thomsen, Isaac P Open Forum Infect Dis Brief Report Improved diagnostics are needed for children with musculoskeletal infections (MSKIs). We assessed the performance of target-enriched multiplex polymerase chain reaction (TEM-PCR) in children with MSKI. TEM-PCR was concordant with culture in pathogen identification and antibiotic susceptibility testing, while increasing the overall yield of pathogen detection. This technology has the potential to inform judicious antimicrobial use early in the disease course. Oxford University Press 2018-05-22 /pmc/articles/PMC6007387/ /pubmed/29977969 http://dx.doi.org/10.1093/ofid/ofy119 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Brief Report
Wood, James B
Sesler, Cheryl
Stalons, Donald
Grigorenko, Elena
Schoenecker, Jonathan G
Creech, C Buddy
Thomsen, Isaac P
Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections
title Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections
title_full Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections
title_fullStr Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections
title_full_unstemmed Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections
title_short Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections
title_sort performance of tem-pcr vs culture for bacterial identification in pediatric musculoskeletal infections
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007387/
https://www.ncbi.nlm.nih.gov/pubmed/29977969
http://dx.doi.org/10.1093/ofid/ofy119
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