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Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize

Multiple centromere misdivision derivatives of a translocation between the supernumerary B chromosome and the short arm of chromosome 9 (TB-9Sb) permit investigation of how centromeres of different sizes behave in meiosis in opposition or in competition with each other. In the first analysis, hetero...

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Autores principales: Han, Fangpu, Lamb, Jonathan C., McCaw, Morgan E., Gao, Zhi, Zhang, Bing, Swyers, Nathan C., Birchler, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008422/
https://www.ncbi.nlm.nih.gov/pubmed/29951076
http://dx.doi.org/10.3389/fpls.2018.00785
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author Han, Fangpu
Lamb, Jonathan C.
McCaw, Morgan E.
Gao, Zhi
Zhang, Bing
Swyers, Nathan C.
Birchler, James A.
author_facet Han, Fangpu
Lamb, Jonathan C.
McCaw, Morgan E.
Gao, Zhi
Zhang, Bing
Swyers, Nathan C.
Birchler, James A.
author_sort Han, Fangpu
collection PubMed
description Multiple centromere misdivision derivatives of a translocation between the supernumerary B chromosome and the short arm of chromosome 9 (TB-9Sb) permit investigation of how centromeres of different sizes behave in meiosis in opposition or in competition with each other. In the first analysis, heterozygotes were produced between the normal TB-9Sb and derivatives of it that resulted from centromere misdivision that reduced the amounts of centromeric DNA. These heterozygotes could test whether these drastic differences would result in meiotic drive of the larger chromosome in female meiosis. Cytological determinations of the segregation of large and small centromeres among thousands of progeny of four combinations were made. The recovery of the larger centromere was at a few percent higher frequency in two of four combinations. However, examination of phosphorylated histone H2A-Thr133, a characteristic of active centromeres, showed a lack of correlation with the size of the centromeric DNA, suggesting an expansion of the basal protein features of the kinetochore in two of the three cases despite the reduction in the size of the underlying DNA. In the second analysis, plants containing different sizes of the B chromosome centromere were crossed to plants with TB-9Sb with a foldback duplication of 9S (TB-9Sb-Dp9). In the progeny, plants containing large and small versions of the B chromosome centromere were selected by FISH. A meiotic “tug of war” occurred in hybrid combinations by recombination between the normal 9S and the foldback duplication in those cases in which pairing occurred. Such pairing and recombination produce anaphase I bridges but in some cases the large and small centromeres progressed to the same pole. In one combination, new dicentric chromosomes were found in the progeny. Collectively, the results indicate that the size of the underlying DNA of a centromere does not dramatically affect its segregation properties or its ability to progress to the poles in meiosis potentially because the biochemical features of centromeres adjust to the cellular conditions.
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spelling pubmed-60084222018-06-27 Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize Han, Fangpu Lamb, Jonathan C. McCaw, Morgan E. Gao, Zhi Zhang, Bing Swyers, Nathan C. Birchler, James A. Front Plant Sci Plant Science Multiple centromere misdivision derivatives of a translocation between the supernumerary B chromosome and the short arm of chromosome 9 (TB-9Sb) permit investigation of how centromeres of different sizes behave in meiosis in opposition or in competition with each other. In the first analysis, heterozygotes were produced between the normal TB-9Sb and derivatives of it that resulted from centromere misdivision that reduced the amounts of centromeric DNA. These heterozygotes could test whether these drastic differences would result in meiotic drive of the larger chromosome in female meiosis. Cytological determinations of the segregation of large and small centromeres among thousands of progeny of four combinations were made. The recovery of the larger centromere was at a few percent higher frequency in two of four combinations. However, examination of phosphorylated histone H2A-Thr133, a characteristic of active centromeres, showed a lack of correlation with the size of the centromeric DNA, suggesting an expansion of the basal protein features of the kinetochore in two of the three cases despite the reduction in the size of the underlying DNA. In the second analysis, plants containing different sizes of the B chromosome centromere were crossed to plants with TB-9Sb with a foldback duplication of 9S (TB-9Sb-Dp9). In the progeny, plants containing large and small versions of the B chromosome centromere were selected by FISH. A meiotic “tug of war” occurred in hybrid combinations by recombination between the normal 9S and the foldback duplication in those cases in which pairing occurred. Such pairing and recombination produce anaphase I bridges but in some cases the large and small centromeres progressed to the same pole. In one combination, new dicentric chromosomes were found in the progeny. Collectively, the results indicate that the size of the underlying DNA of a centromere does not dramatically affect its segregation properties or its ability to progress to the poles in meiosis potentially because the biochemical features of centromeres adjust to the cellular conditions. Frontiers Media S.A. 2018-06-13 /pmc/articles/PMC6008422/ /pubmed/29951076 http://dx.doi.org/10.3389/fpls.2018.00785 Text en Copyright © 2018 Han, Lamb, McCaw, Gao, Zhang, Swyers and Birchler. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Han, Fangpu
Lamb, Jonathan C.
McCaw, Morgan E.
Gao, Zhi
Zhang, Bing
Swyers, Nathan C.
Birchler, James A.
Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize
title Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize
title_full Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize
title_fullStr Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize
title_full_unstemmed Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize
title_short Meiotic Studies on Combinations of Chromosomes With Different Sized Centromeres in Maize
title_sort meiotic studies on combinations of chromosomes with different sized centromeres in maize
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008422/
https://www.ncbi.nlm.nih.gov/pubmed/29951076
http://dx.doi.org/10.3389/fpls.2018.00785
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