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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing
Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within ca...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Scientific Pub. Co
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008488/ https://www.ncbi.nlm.nih.gov/pubmed/29885806 http://dx.doi.org/10.1016/j.vetmic.2018.05.011 |
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| author | Bossé, Janine T. Li, Yanwen Fernandez Crespo, Roberto Lacouture, Sonia Gottschalk, Marcelo Sárközi, Rita Fodor, László Casas Amoribieta, Maria Angen, Øystein Nedbalcova, Katerina Holden, Matthew T.G. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. |
| author_facet | Bossé, Janine T. Li, Yanwen Fernandez Crespo, Roberto Lacouture, Sonia Gottschalk, Marcelo Sárközi, Rita Fodor, László Casas Amoribieta, Maria Angen, Øystein Nedbalcova, Katerina Holden, Matthew T.G. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. |
| author_sort | Bossé, Janine T. |
| collection | PubMed |
| description | Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen. |
| format | Online Article Text |
| id | pubmed-6008488 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | Elsevier Scientific Pub. Co |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-60084882018-07-01 Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing Bossé, Janine T. Li, Yanwen Fernandez Crespo, Roberto Lacouture, Sonia Gottschalk, Marcelo Sárközi, Rita Fodor, László Casas Amoribieta, Maria Angen, Øystein Nedbalcova, Katerina Holden, Matthew T.G. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. Vet Microbiol Article Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen. Elsevier Scientific Pub. Co 2018-07 /pmc/articles/PMC6008488/ /pubmed/29885806 http://dx.doi.org/10.1016/j.vetmic.2018.05.011 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Bossé, Janine T. Li, Yanwen Fernandez Crespo, Roberto Lacouture, Sonia Gottschalk, Marcelo Sárközi, Rita Fodor, László Casas Amoribieta, Maria Angen, Øystein Nedbalcova, Katerina Holden, Matthew T.G. Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing |
| title | Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing |
| title_full | Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing |
| title_fullStr | Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing |
| title_full_unstemmed | Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing |
| title_short | Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex PCRs for comprehensive capsule typing |
| title_sort | comparative sequence analysis of the capsular polysaccharide loci of actinobacillus pleuropneumoniae serovars 1–18, and development of two multiplex pcrs for comprehensive capsule typing |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008488/ https://www.ncbi.nlm.nih.gov/pubmed/29885806 http://dx.doi.org/10.1016/j.vetmic.2018.05.011 |
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