Proteomic analysis of a Candida albicans pga1 Null Strain

We previously characterized Pga1, a Candida albicans (C. albicans) cell wall protein necessary for proper virulence, adhesion, and resistance to oxidative stress. By utilizing tandem mass spectrometry coupled with bioinformatics to investigate cell wall proteome expression in a pga1 null fourteen an...

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Detalles Bibliográficos
Autores principales: Awad, Andy, El Khoury, Pamela, Wex, Brigitte, Khalaf, Roy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008633/
https://www.ncbi.nlm.nih.gov/pubmed/29928583
http://dx.doi.org/10.1016/j.euprot.2018.02.001
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author Awad, Andy
El Khoury, Pamela
Wex, Brigitte
Khalaf, Roy A.
author_facet Awad, Andy
El Khoury, Pamela
Wex, Brigitte
Khalaf, Roy A.
author_sort Awad, Andy
collection PubMed
description We previously characterized Pga1, a Candida albicans (C. albicans) cell wall protein necessary for proper virulence, adhesion, and resistance to oxidative stress. By utilizing tandem mass spectrometry coupled with bioinformatics to investigate cell wall proteome expression in a pga1 null fourteen and 36 proteins were identified in the wild type grown under filamentous and non-filamentous conditions respectively, but were not detected in the mutant, including members of the PGA GPI anchored family. Virulence and adhesion proteins such as Hsp 90, Sap10, Cdc11, Int 3 and members of the lipase family were also identified exclusively in the wild type.
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spelling pubmed-60086332018-06-20 Proteomic analysis of a Candida albicans pga1 Null Strain Awad, Andy El Khoury, Pamela Wex, Brigitte Khalaf, Roy A. EuPA Open Proteom Article We previously characterized Pga1, a Candida albicans (C. albicans) cell wall protein necessary for proper virulence, adhesion, and resistance to oxidative stress. By utilizing tandem mass spectrometry coupled with bioinformatics to investigate cell wall proteome expression in a pga1 null fourteen and 36 proteins were identified in the wild type grown under filamentous and non-filamentous conditions respectively, but were not detected in the mutant, including members of the PGA GPI anchored family. Virulence and adhesion proteins such as Hsp 90, Sap10, Cdc11, Int 3 and members of the lipase family were also identified exclusively in the wild type. Elsevier 2018-02-22 /pmc/articles/PMC6008633/ /pubmed/29928583 http://dx.doi.org/10.1016/j.euprot.2018.02.001 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Awad, Andy
El Khoury, Pamela
Wex, Brigitte
Khalaf, Roy A.
Proteomic analysis of a Candida albicans pga1 Null Strain
title Proteomic analysis of a Candida albicans pga1 Null Strain
title_full Proteomic analysis of a Candida albicans pga1 Null Strain
title_fullStr Proteomic analysis of a Candida albicans pga1 Null Strain
title_full_unstemmed Proteomic analysis of a Candida albicans pga1 Null Strain
title_short Proteomic analysis of a Candida albicans pga1 Null Strain
title_sort proteomic analysis of a candida albicans pga1 null strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008633/
https://www.ncbi.nlm.nih.gov/pubmed/29928583
http://dx.doi.org/10.1016/j.euprot.2018.02.001
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