Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier

After a combined enzymic and mechanical disruption of the spinal cord tissue, the low-density motoneurons band at the interface of a 1.06-g/ml barrier through which other contaminating cells sediment.

Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009274/
https://www.ncbi.nlm.nih.gov/pubmed/12806137
http://dx.doi.org/10.1100/tsw.2002.841
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author Graham, John
author_facet Graham, John
author_sort Graham, John
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description After a combined enzymic and mechanical disruption of the spinal cord tissue, the low-density motoneurons band at the interface of a 1.06-g/ml barrier through which other contaminating cells sediment.
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spelling pubmed-60092742018-07-04 Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier Graham, John ScientificWorldJournal Peer-Reviewed Protocol After a combined enzymic and mechanical disruption of the spinal cord tissue, the low-density motoneurons band at the interface of a 1.06-g/ml barrier through which other contaminating cells sediment. TheScientificWorldJOURNAL 2002-06-07 /pmc/articles/PMC6009274/ /pubmed/12806137 http://dx.doi.org/10.1100/tsw.2002.841 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
title Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
title_full Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
title_fullStr Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
title_full_unstemmed Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
title_short Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
title_sort isolation of a mouse motoneuron-enriched fraction from mouse spinal cord on a density barrier
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009274/
https://www.ncbi.nlm.nih.gov/pubmed/12806137
http://dx.doi.org/10.1100/tsw.2002.841
work_keys_str_mv AT grahamjohn isolationofamousemotoneuronenrichedfractionfrommousespinalcordonadensitybarrier

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