Purification of Intact Plant Protoplasts by Flotation at 1g

From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.

Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009277/
https://www.ncbi.nlm.nih.gov/pubmed/12805924
http://dx.doi.org/10.1100/tsw.2002.835
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author Graham, John
author_facet Graham, John
author_sort Graham, John
collection PubMed
description From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.
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spelling pubmed-60092772018-07-04 Purification of Intact Plant Protoplasts by Flotation at 1g Graham, John ScientificWorldJournal Peer-Reviewed Protocol From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g. TheScientificWorldJOURNAL 2002-05-21 /pmc/articles/PMC6009277/ /pubmed/12805924 http://dx.doi.org/10.1100/tsw.2002.835 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Purification of Intact Plant Protoplasts by Flotation at 1g
title Purification of Intact Plant Protoplasts by Flotation at 1g
title_full Purification of Intact Plant Protoplasts by Flotation at 1g
title_fullStr Purification of Intact Plant Protoplasts by Flotation at 1g
title_full_unstemmed Purification of Intact Plant Protoplasts by Flotation at 1g
title_short Purification of Intact Plant Protoplasts by Flotation at 1g
title_sort purification of intact plant protoplasts by flotation at 1g
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009277/
https://www.ncbi.nlm.nih.gov/pubmed/12805924
http://dx.doi.org/10.1100/tsw.2002.835
work_keys_str_mv AT grahamjohn purificationofintactplantprotoplastsbyflotationat1g

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