Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the...

Descripción completa

Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009316/
https://www.ncbi.nlm.nih.gov/pubmed/12806154
http://dx.doi.org/10.1100/tsw.2002.852
_version_ 1783333355143561216
author Graham, John
author_facet Graham, John
author_sort Graham, John
collection PubMed
description Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.
format Online
Article
Text
id pubmed-6009316
institution National Center for Biotechnology Information
language English
publishDate 2002
publisher TheScientificWorldJOURNAL
record_format MEDLINE/PubMed
spelling pubmed-60093162018-07-04 Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction Graham, John ScientificWorldJournal Peer-Reviewed Protocol Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation. TheScientificWorldJOURNAL 2002-06-14 /pmc/articles/PMC6009316/ /pubmed/12806154 http://dx.doi.org/10.1100/tsw.2002.852 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
title Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
title_full Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
title_fullStr Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
title_full_unstemmed Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
title_short Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
title_sort purification of gastric mucosal ecl cells from a crude elutriation fraction
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009316/
https://www.ncbi.nlm.nih.gov/pubmed/12806154
http://dx.doi.org/10.1100/tsw.2002.852
work_keys_str_mv AT grahamjohn purificationofgastricmucosaleclcellsfromacrudeelutriationfraction

Ejemplares similares