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Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient
The plating efficiency of neurons in culture is highly dependent on the concentration of cells used to establish the monolayer. A discontinuous iodixanol gradient permits both the production of a viable concentrated suspension of neurons and purification from other brain tissue elements. The gradien...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009317/ https://www.ncbi.nlm.nih.gov/pubmed/12806152 http://dx.doi.org/10.1100/tsw.2002.850 |