Purification of Parietal and Chief Cells from the Gastric Mucosa

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport. A discontinuous gradient of iodixanol has been found to be superior to earlier protocols using Nycodenz® and this method, which removes a significant amount of contaminating cells and muc...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009405/
https://www.ncbi.nlm.nih.gov/pubmed/12806156
http://dx.doi.org/10.1100/tsw.2002.853
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author Graham, John
author_facet Graham, John
author_sort Graham, John
collection PubMed
description Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport. A discontinuous gradient of iodixanol has been found to be superior to earlier protocols using Nycodenz® and this method, which removes a significant amount of contaminating cells and mucus is a very useful prelude to further purification by elutriation.
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spelling pubmed-60094052018-07-04 Purification of Parietal and Chief Cells from the Gastric Mucosa Graham, John ScientificWorldJournal Peer-Reviewed Protocol Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport. A discontinuous gradient of iodixanol has been found to be superior to earlier protocols using Nycodenz® and this method, which removes a significant amount of contaminating cells and mucus is a very useful prelude to further purification by elutriation. TheScientificWorldJOURNAL 2002-06-15 /pmc/articles/PMC6009405/ /pubmed/12806156 http://dx.doi.org/10.1100/tsw.2002.853 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Purification of Parietal and Chief Cells from the Gastric Mucosa
title Purification of Parietal and Chief Cells from the Gastric Mucosa
title_full Purification of Parietal and Chief Cells from the Gastric Mucosa
title_fullStr Purification of Parietal and Chief Cells from the Gastric Mucosa
title_full_unstemmed Purification of Parietal and Chief Cells from the Gastric Mucosa
title_short Purification of Parietal and Chief Cells from the Gastric Mucosa
title_sort purification of parietal and chief cells from the gastric mucosa
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009405/
https://www.ncbi.nlm.nih.gov/pubmed/12806156
http://dx.doi.org/10.1100/tsw.2002.853
work_keys_str_mv AT grahamjohn purificationofparietalandchiefcellsfromthegastricmucosa

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