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Formation of Self-Generated Gradients of Iodixanol
The formation of self-generated gradients of iodixanol from a solution of uniform concentration requires the use of vertical or near-vertical rotors. The density profile that is generated depends upon the sedimentation path length of the rotor, centrifugation time, RCF and temperature. Modulation of...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009428/ https://www.ncbi.nlm.nih.gov/pubmed/12805920 http://dx.doi.org/10.1100/tsw.2002.284 |
| _version_ | 1783333382419120128 |
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| author | Graham, John |
| author_facet | Graham, John |
| author_sort | Graham, John |
| collection | PubMed |
| description | The formation of self-generated gradients of iodixanol from a solution of uniform concentration requires the use of vertical or near-vertical rotors. The density profile that is generated depends upon the sedimentation path length of the rotor, centrifugation time, RCF and temperature. Modulation of the starting concentration changes the density range of the gradient. This Protocol Article illustrates the effect of these parameters on gradient shape in a few selected rotors. Because the gradients are formed by the centrifugal field, they are highly reproducible and easy to execute. |
| format | Online Article Text |
| id | pubmed-6009428 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2002 |
| publisher | TheScientificWorldJOURNAL |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-60094282018-07-04 Formation of Self-Generated Gradients of Iodixanol Graham, John ScientificWorldJournal Peer-Reviewed Protocol The formation of self-generated gradients of iodixanol from a solution of uniform concentration requires the use of vertical or near-vertical rotors. The density profile that is generated depends upon the sedimentation path length of the rotor, centrifugation time, RCF and temperature. Modulation of the starting concentration changes the density range of the gradient. This Protocol Article illustrates the effect of these parameters on gradient shape in a few selected rotors. Because the gradients are formed by the centrifugal field, they are highly reproducible and easy to execute. TheScientificWorldJOURNAL 2002-05-16 /pmc/articles/PMC6009428/ /pubmed/12805920 http://dx.doi.org/10.1100/tsw.2002.284 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Peer-Reviewed Protocol Graham, John Formation of Self-Generated Gradients of Iodixanol |
| title | Formation of Self-Generated Gradients of Iodixanol |
| title_full | Formation of Self-Generated Gradients of Iodixanol |
| title_fullStr | Formation of Self-Generated Gradients of Iodixanol |
| title_full_unstemmed | Formation of Self-Generated Gradients of Iodixanol |
| title_short | Formation of Self-Generated Gradients of Iodixanol |
| title_sort | formation of self-generated gradients of iodixanol |
| topic | Peer-Reviewed Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009428/ https://www.ncbi.nlm.nih.gov/pubmed/12805920 http://dx.doi.org/10.1100/tsw.2002.284 |
| work_keys_str_mv | AT grahamjohn formationofselfgeneratedgradientsofiodixanol |