Separation of Monocytes from Whole Human Blood

Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The m...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009477/
https://www.ncbi.nlm.nih.gov/pubmed/12806136
http://dx.doi.org/10.1100/tsw.2002.842
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author Graham, John
author_facet Graham, John
author_sort Graham, John
collection PubMed
description Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood. Preliminary evidence suggests that this technique may be applicable to blood from rats.
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spelling pubmed-60094772018-07-04 Separation of Monocytes from Whole Human Blood Graham, John ScientificWorldJournal Peer-Reviewed Protocol Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood. Preliminary evidence suggests that this technique may be applicable to blood from rats. TheScientificWorldJOURNAL 2002-06-07 /pmc/articles/PMC6009477/ /pubmed/12806136 http://dx.doi.org/10.1100/tsw.2002.842 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Separation of Monocytes from Whole Human Blood
title Separation of Monocytes from Whole Human Blood
title_full Separation of Monocytes from Whole Human Blood
title_fullStr Separation of Monocytes from Whole Human Blood
title_full_unstemmed Separation of Monocytes from Whole Human Blood
title_short Separation of Monocytes from Whole Human Blood
title_sort separation of monocytes from whole human blood
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009477/
https://www.ncbi.nlm.nih.gov/pubmed/12806136
http://dx.doi.org/10.1100/tsw.2002.842
work_keys_str_mv AT grahamjohn separationofmonocytesfromwholehumanblood

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