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Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient
A continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009484/ https://www.ncbi.nlm.nih.gov/pubmed/12805929 http://dx.doi.org/10.1100/tsw.2002.286 |
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author | Graham, John |
author_facet | Graham, John |
author_sort | Graham, John |
collection | PubMed |
description | A continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and the centrifugation conditions (100,000-200,000 g for 2-16 h) vary with the type of cell and the requirements of the separation. The strategy is widely used to study the processing of proteins within cells. |
format | Online Article Text |
id | pubmed-6009484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | TheScientificWorldJOURNAL |
record_format | MEDLINE/PubMed |
spelling | pubmed-60094842018-07-04 Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient Graham, John ScientificWorldJournal Peer-Reviewed Protocol A continuous iodixanol gradient within the range 0-30% (w/v) iodixanol can resolve the major membrane compartments of the endoplasmic reticulum, Golgi membranes, and plasma membrane from a postnuclear supernatant prepared from a cultured cell homogenate. The precise density range of the gradient and the centrifugation conditions (100,000-200,000 g for 2-16 h) vary with the type of cell and the requirements of the separation. The strategy is widely used to study the processing of proteins within cells. TheScientificWorldJOURNAL 2002-05-22 /pmc/articles/PMC6009484/ /pubmed/12805929 http://dx.doi.org/10.1100/tsw.2002.286 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Peer-Reviewed Protocol Graham, John Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
title | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
title_full | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
title_fullStr | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
title_full_unstemmed | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
title_short | Fractionation of Golgi, Endoplasmic Reticulum, and Plasma Membrane from Cultured Cells in a Preformed Continuous Iodixanol Gradient |
title_sort | fractionation of golgi, endoplasmic reticulum, and plasma membrane from cultured cells in a preformed continuous iodixanol gradient |
topic | Peer-Reviewed Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009484/ https://www.ncbi.nlm.nih.gov/pubmed/12805929 http://dx.doi.org/10.1100/tsw.2002.286 |
work_keys_str_mv | AT grahamjohn fractionationofgolgiendoplasmicreticulumandplasmamembranefromculturedcellsinapreformedcontinuousiodixanolgradient |