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Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection
More than 140 post-transcriptional modifications (PTMs) are known to decorate cellular RNAs, but their incidence, identity and significance in viral RNA are still largely unknown. We have developed an agnostic analytical approach to comprehensively survey PTMs on viral and cellular RNAs. Specificall...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009648/ https://www.ncbi.nlm.nih.gov/pubmed/29373715 http://dx.doi.org/10.1093/nar/gky029 |
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author | McIntyre, Will Netzband, Rachel Bonenfant, Gaston Biegel, Jason M Miller, Clare Fuchs, Gabriele Henderson, Eric Arra, Manoj Canki, Mario Fabris, Daniele Pager, Cara T |
author_facet | McIntyre, Will Netzband, Rachel Bonenfant, Gaston Biegel, Jason M Miller, Clare Fuchs, Gabriele Henderson, Eric Arra, Manoj Canki, Mario Fabris, Daniele Pager, Cara T |
author_sort | McIntyre, Will |
collection | PubMed |
description | More than 140 post-transcriptional modifications (PTMs) are known to decorate cellular RNAs, but their incidence, identity and significance in viral RNA are still largely unknown. We have developed an agnostic analytical approach to comprehensively survey PTMs on viral and cellular RNAs. Specifically, we used mass spectrometry to analyze PTMs on total RNA isolated from cells infected with Zika virus, Dengue virus, hepatitis C virus (HCV), poliovirus and human immunodeficiency virus type 1. All five RNA viruses significantly altered global PTM landscapes. Examination of PTM profiles of individual viral genomes isolated by affinity capture revealed a plethora of PTMs on viral RNAs, which far exceeds the handful of well-characterized modifications. Direct comparison of viral epitranscriptomes identified common and virus-specific PTMs. In particular, specific dimethylcytosine modifications were only present in total RNA from virus-infected cells, and in intracellular HCV RNA, and viral RNA from Zika and HCV virions. Moreover, dimethylcytosine abundance during viral infection was modulated by the cellular DEAD-box RNA helicase DDX6. By opening the Pandora’s box on viral PTMs, this report presents numerous questions and hypotheses on PTM function and strongly supports PTMs as a new tier of regulation by which RNA viruses subvert the host and evade cellular surveillance systems. |
format | Online Article Text |
id | pubmed-6009648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60096482018-06-25 Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection McIntyre, Will Netzband, Rachel Bonenfant, Gaston Biegel, Jason M Miller, Clare Fuchs, Gabriele Henderson, Eric Arra, Manoj Canki, Mario Fabris, Daniele Pager, Cara T Nucleic Acids Res RNA and RNA-protein complexes More than 140 post-transcriptional modifications (PTMs) are known to decorate cellular RNAs, but their incidence, identity and significance in viral RNA are still largely unknown. We have developed an agnostic analytical approach to comprehensively survey PTMs on viral and cellular RNAs. Specifically, we used mass spectrometry to analyze PTMs on total RNA isolated from cells infected with Zika virus, Dengue virus, hepatitis C virus (HCV), poliovirus and human immunodeficiency virus type 1. All five RNA viruses significantly altered global PTM landscapes. Examination of PTM profiles of individual viral genomes isolated by affinity capture revealed a plethora of PTMs on viral RNAs, which far exceeds the handful of well-characterized modifications. Direct comparison of viral epitranscriptomes identified common and virus-specific PTMs. In particular, specific dimethylcytosine modifications were only present in total RNA from virus-infected cells, and in intracellular HCV RNA, and viral RNA from Zika and HCV virions. Moreover, dimethylcytosine abundance during viral infection was modulated by the cellular DEAD-box RNA helicase DDX6. By opening the Pandora’s box on viral PTMs, this report presents numerous questions and hypotheses on PTM function and strongly supports PTMs as a new tier of regulation by which RNA viruses subvert the host and evade cellular surveillance systems. Oxford University Press 2018-06-20 2018-01-24 /pmc/articles/PMC6009648/ /pubmed/29373715 http://dx.doi.org/10.1093/nar/gky029 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | RNA and RNA-protein complexes McIntyre, Will Netzband, Rachel Bonenfant, Gaston Biegel, Jason M Miller, Clare Fuchs, Gabriele Henderson, Eric Arra, Manoj Canki, Mario Fabris, Daniele Pager, Cara T Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
title | Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
title_full | Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
title_fullStr | Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
title_full_unstemmed | Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
title_short | Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
title_sort | positive-sense rna viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection |
topic | RNA and RNA-protein complexes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009648/ https://www.ncbi.nlm.nih.gov/pubmed/29373715 http://dx.doi.org/10.1093/nar/gky029 |
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