Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier

The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also...

Descripción completa

Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009712/
https://www.ncbi.nlm.nih.gov/pubmed/12806157
http://dx.doi.org/10.1100/tsw.2002.845
_version_ 1783333449670590464
author Graham, John
author_facet Graham, John
author_sort Graham, John
collection PubMed
description The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose (Ficoll) with a density of approx 1.074 g/ml.
format Online
Article
Text
id pubmed-6009712
institution National Center for Biotechnology Information
language English
publishDate 2002
publisher TheScientificWorldJOURNAL
record_format MEDLINE/PubMed
spelling pubmed-60097122018-07-04 Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier Graham, John ScientificWorldJournal Peer-Reviewed Protocol The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose (Ficoll) with a density of approx 1.074 g/ml. TheScientificWorldJOURNAL 2002-06-15 /pmc/articles/PMC6009712/ /pubmed/12806157 http://dx.doi.org/10.1100/tsw.2002.845 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_full Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_fullStr Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_full_unstemmed Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_short Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier
title_sort isolation of peripheral blood mononuclear cells from macaques on a density barrier
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009712/
https://www.ncbi.nlm.nih.gov/pubmed/12806157
http://dx.doi.org/10.1100/tsw.2002.845
work_keys_str_mv AT grahamjohn isolationofperipheralbloodmononuclearcellsfrommacaquesonadensitybarrier

Ejemplares similares