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Preparation of Crude Subcellular Fractions by Differential Centrifugation

The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient. Buoyant density gradient purification of peroxisomes or lysosomes for exampl...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009713/
https://www.ncbi.nlm.nih.gov/pubmed/12806153
http://dx.doi.org/10.1100/tsw.2002.851
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author Graham, John
author_facet Graham, John
author_sort Graham, John
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description The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient. Buoyant density gradient purification of peroxisomes or lysosomes for example is almost invariably carried out on a light mitochondrial fraction so as to eliminate smaller particles that may have similar densities. Unless they are first removed, large rapidly sedimenting particles in homogenates may also disturb shallow gradients designed to fractionate small low-density microsomes.
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spelling pubmed-60097132018-07-04 Preparation of Crude Subcellular Fractions by Differential Centrifugation Graham, John ScientificWorldJournal Peer-Reviewed Protocol The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient. Buoyant density gradient purification of peroxisomes or lysosomes for example is almost invariably carried out on a light mitochondrial fraction so as to eliminate smaller particles that may have similar densities. Unless they are first removed, large rapidly sedimenting particles in homogenates may also disturb shallow gradients designed to fractionate small low-density microsomes. TheScientificWorldJOURNAL 2002-06-13 /pmc/articles/PMC6009713/ /pubmed/12806153 http://dx.doi.org/10.1100/tsw.2002.851 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Preparation of Crude Subcellular Fractions by Differential Centrifugation
title Preparation of Crude Subcellular Fractions by Differential Centrifugation
title_full Preparation of Crude Subcellular Fractions by Differential Centrifugation
title_fullStr Preparation of Crude Subcellular Fractions by Differential Centrifugation
title_full_unstemmed Preparation of Crude Subcellular Fractions by Differential Centrifugation
title_short Preparation of Crude Subcellular Fractions by Differential Centrifugation
title_sort preparation of crude subcellular fractions by differential centrifugation
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009713/
https://www.ncbi.nlm.nih.gov/pubmed/12806153
http://dx.doi.org/10.1100/tsw.2002.851
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