Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling

Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also,...

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Detalles Bibliográficos
Autores principales: Lobo, C., Sanchez, M., Garbi, C., Ferrer, E., Martinez-Iñigo, M.J., Allende, J.L., Martinez, C., Casasas, L., Alonso, R., Gibello, A., Martin, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009742/
https://www.ncbi.nlm.nih.gov/pubmed/12805921
http://dx.doi.org/10.1100/tsw.2002.211
Descripción
Sumario:Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation.

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