Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling

Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also,...

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Autores principales: Lobo, C., Sanchez, M., Garbi, C., Ferrer, E., Martinez-Iñigo, M.J., Allende, J.L., Martinez, C., Casasas, L., Alonso, R., Gibello, A., Martin, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009742/
https://www.ncbi.nlm.nih.gov/pubmed/12805921
http://dx.doi.org/10.1100/tsw.2002.211
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author Lobo, C.
Sanchez, M.
Garbi, C.
Ferrer, E.
Martinez-Iñigo, M.J.
Allende, J.L.
Martinez, C.
Casasas, L.
Alonso, R.
Gibello, A.
Martin, M.
author_facet Lobo, C.
Sanchez, M.
Garbi, C.
Ferrer, E.
Martinez-Iñigo, M.J.
Allende, J.L.
Martinez, C.
Casasas, L.
Alonso, R.
Gibello, A.
Martin, M.
author_sort Lobo, C.
collection PubMed
description Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation.
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spelling pubmed-60097422018-07-04 Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling Lobo, C. Sanchez, M. Garbi, C. Ferrer, E. Martinez-Iñigo, M.J. Allende, J.L. Martinez, C. Casasas, L. Alonso, R. Gibello, A. Martin, M. ScientificWorldJournal Short Communication Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound—degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation. TheScientificWorldJOURNAL 2002-05-18 /pmc/articles/PMC6009742/ /pubmed/12805921 http://dx.doi.org/10.1100/tsw.2002.211 Text en Copyright © 2002 C. Lobo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Lobo, C.
Sanchez, M.
Garbi, C.
Ferrer, E.
Martinez-Iñigo, M.J.
Allende, J.L.
Martinez, C.
Casasas, L.
Alonso, R.
Gibello, A.
Martin, M.
Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
title Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
title_full Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
title_fullStr Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
title_full_unstemmed Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
title_short Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling
title_sort immobilized native bacteria as a tool for bioremediation of soils and waters: implementation and modeling
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009742/
https://www.ncbi.nlm.nih.gov/pubmed/12805921
http://dx.doi.org/10.1100/tsw.2002.211
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