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Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development

Fructans are fructose-based oligo- and polymers that serve as reserve carbohydrates in many plant species. The biochemistry of fructan biosynthesis in dicots has been resolved, and the respective cDNAs have been cloned. Recent progress has now succeeded in elucidating the biochemistry and molecular...

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Autores principales: Van den Ende, Wim, Michiels, An, De Roover, Joke, Van Laere, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009745/
https://www.ncbi.nlm.nih.gov/pubmed/12805911
http://dx.doi.org/10.1100/tsw.2002.288
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author Van den Ende, Wim
Michiels, An
De Roover, Joke
Van Laere, Andrea
author_facet Van den Ende, Wim
Michiels, An
De Roover, Joke
Van Laere, Andrea
author_sort Van den Ende, Wim
collection PubMed
description Fructans are fructose-based oligo- and polymers that serve as reserve carbohydrates in many plant species. The biochemistry of fructan biosynthesis in dicots has been resolved, and the respective cDNAs have been cloned. Recent progress has now succeeded in elucidating the biochemistry and molecular biology of fructan biodegradation in chicory, an economically important species used for commercial inulin extraction. Unlike fructan biosynthetic genes that originated from vacuolar-type invertase, fructan exohydrolases (FEHs) seem to have evolved from a cell-wall invertase ancestor gene that later obtained a low iso-electric point and a vacuolar targeting signal. Expression analysis reveals that fructan enzymes are controlled mainly at the transcriptional level. Using chicory as a model system, northern analysis was consistent with enzymatic activity measurements and observed carbohydrate changes throughout its development.
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spelling pubmed-60097452018-07-04 Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development Van den Ende, Wim Michiels, An De Roover, Joke Van Laere, Andrea ScientificWorldJournal Mini-Review Article Fructans are fructose-based oligo- and polymers that serve as reserve carbohydrates in many plant species. The biochemistry of fructan biosynthesis in dicots has been resolved, and the respective cDNAs have been cloned. Recent progress has now succeeded in elucidating the biochemistry and molecular biology of fructan biodegradation in chicory, an economically important species used for commercial inulin extraction. Unlike fructan biosynthetic genes that originated from vacuolar-type invertase, fructan exohydrolases (FEHs) seem to have evolved from a cell-wall invertase ancestor gene that later obtained a low iso-electric point and a vacuolar targeting signal. Expression analysis reveals that fructan enzymes are controlled mainly at the transcriptional level. Using chicory as a model system, northern analysis was consistent with enzymatic activity measurements and observed carbohydrate changes throughout its development. TheScientificWorldJOURNAL 2002-05-11 /pmc/articles/PMC6009745/ /pubmed/12805911 http://dx.doi.org/10.1100/tsw.2002.288 Text en Copyright © 2002 Wim Van den Ende et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mini-Review Article
Van den Ende, Wim
Michiels, An
De Roover, Joke
Van Laere, Andrea
Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development
title Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development
title_full Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development
title_fullStr Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development
title_full_unstemmed Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development
title_short Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development
title_sort fructan biosynthetic and breakdown enzymes in dicots evolved from different invertases. expression of fructan genes throughout chicory development
topic Mini-Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009745/
https://www.ncbi.nlm.nih.gov/pubmed/12805911
http://dx.doi.org/10.1100/tsw.2002.288
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