Optimal 3D single-molecule localization in real time using experimental point spread functions

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution u...

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Detalles Bibliográficos
Autores principales: Li, Yiming, Mund, Markus, Hoess, Philipp, Deschamps, Joran, Matti, Ulf, Nijmeijer, Bianca, Sabinina, Vilma Jimenez, Ellenberg, Jan, Schoen, Ingmar, Ries, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009849/
https://www.ncbi.nlm.nih.gov/pubmed/29630062
http://dx.doi.org/10.1038/nmeth.4661
Descripción
Sumario:We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution unprecedented for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D superresolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.

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