Optimal 3D single-molecule localization in real time using experimental point spread functions

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution u...

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Autores principales: Li, Yiming, Mund, Markus, Hoess, Philipp, Deschamps, Joran, Matti, Ulf, Nijmeijer, Bianca, Sabinina, Vilma Jimenez, Ellenberg, Jan, Schoen, Ingmar, Ries, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009849/
https://www.ncbi.nlm.nih.gov/pubmed/29630062
http://dx.doi.org/10.1038/nmeth.4661
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author Li, Yiming
Mund, Markus
Hoess, Philipp
Deschamps, Joran
Matti, Ulf
Nijmeijer, Bianca
Sabinina, Vilma Jimenez
Ellenberg, Jan
Schoen, Ingmar
Ries, Jonas
author_facet Li, Yiming
Mund, Markus
Hoess, Philipp
Deschamps, Joran
Matti, Ulf
Nijmeijer, Bianca
Sabinina, Vilma Jimenez
Ellenberg, Jan
Schoen, Ingmar
Ries, Jonas
author_sort Li, Yiming
collection PubMed
description We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution unprecedented for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D superresolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.
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spelling pubmed-60098492018-10-09 Optimal 3D single-molecule localization in real time using experimental point spread functions Li, Yiming Mund, Markus Hoess, Philipp Deschamps, Joran Matti, Ulf Nijmeijer, Bianca Sabinina, Vilma Jimenez Ellenberg, Jan Schoen, Ingmar Ries, Jonas Nat Methods Article We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution unprecedented for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D superresolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics. 2018-04-09 2018-05 /pmc/articles/PMC6009849/ /pubmed/29630062 http://dx.doi.org/10.1038/nmeth.4661 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Li, Yiming
Mund, Markus
Hoess, Philipp
Deschamps, Joran
Matti, Ulf
Nijmeijer, Bianca
Sabinina, Vilma Jimenez
Ellenberg, Jan
Schoen, Ingmar
Ries, Jonas
Optimal 3D single-molecule localization in real time using experimental point spread functions
title Optimal 3D single-molecule localization in real time using experimental point spread functions
title_full Optimal 3D single-molecule localization in real time using experimental point spread functions
title_fullStr Optimal 3D single-molecule localization in real time using experimental point spread functions
title_full_unstemmed Optimal 3D single-molecule localization in real time using experimental point spread functions
title_short Optimal 3D single-molecule localization in real time using experimental point spread functions
title_sort optimal 3d single-molecule localization in real time using experimental point spread functions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009849/
https://www.ncbi.nlm.nih.gov/pubmed/29630062
http://dx.doi.org/10.1038/nmeth.4661
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