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Optimal 3D single-molecule localization in real time using experimental point spread functions
We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution u...
Autores principales: | Li, Yiming, Mund, Markus, Hoess, Philipp, Deschamps, Joran, Matti, Ulf, Nijmeijer, Bianca, Sabinina, Vilma Jimenez, Ellenberg, Jan, Schoen, Ingmar, Ries, Jonas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009849/ https://www.ncbi.nlm.nih.gov/pubmed/29630062 http://dx.doi.org/10.1038/nmeth.4661 |
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