Cargando…
A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs
2,2-Diphenyl-1-picrylhydrazyl (DPPH(•)) radical scavenging, the most commonly used antioxidant method with more than seventeen thousand articles cited, is very practical; however, as with most assays, it has the major disadvantage of dependence on a spectrophotometer. To overcome this drawback, the...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009954/ https://www.ncbi.nlm.nih.gov/pubmed/28262029 http://dx.doi.org/10.1080/14756366.2017.1284068 |
_version_ | 1783333498465026048 |
---|---|
author | Akar, Zeynep Küçük, Murat Doğan, Hacer |
author_facet | Akar, Zeynep Küçük, Murat Doğan, Hacer |
author_sort | Akar, Zeynep |
collection | PubMed |
description | 2,2-Diphenyl-1-picrylhydrazyl (DPPH(•)) radical scavenging, the most commonly used antioxidant method with more than seventeen thousand articles cited, is very practical; however, as with most assays, it has the major disadvantage of dependence on a spectrophotometer. To overcome this drawback, the colorimetric determination of the antioxidant activity using a scanner and freely available Image J software was developed. In this new method, the mixtures of solutions of DPPH(•) and standard antioxidants or extracts of common medicinal herbs were dropped onto TLC plates, after an incubation period. The spot images were evaluated with Image J software to determine CSC(50) values, the sample concentrations providing 50% colour reduction, which were very similar with the SC(50) values obtained with spectrophotometric method. The advantages of the new method are the use of lower amounts of reagents and solvents, no need for costly spectrophotometers, and thus significantly lowered costs, and convenient implementation in any environment and situation. |
format | Online Article Text |
id | pubmed-6009954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-60099542018-07-11 A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs Akar, Zeynep Küçük, Murat Doğan, Hacer J Enzyme Inhib Med Chem Research Article 2,2-Diphenyl-1-picrylhydrazyl (DPPH(•)) radical scavenging, the most commonly used antioxidant method with more than seventeen thousand articles cited, is very practical; however, as with most assays, it has the major disadvantage of dependence on a spectrophotometer. To overcome this drawback, the colorimetric determination of the antioxidant activity using a scanner and freely available Image J software was developed. In this new method, the mixtures of solutions of DPPH(•) and standard antioxidants or extracts of common medicinal herbs were dropped onto TLC plates, after an incubation period. The spot images were evaluated with Image J software to determine CSC(50) values, the sample concentrations providing 50% colour reduction, which were very similar with the SC(50) values obtained with spectrophotometric method. The advantages of the new method are the use of lower amounts of reagents and solvents, no need for costly spectrophotometers, and thus significantly lowered costs, and convenient implementation in any environment and situation. Taylor & Francis 2017-03-06 /pmc/articles/PMC6009954/ /pubmed/28262029 http://dx.doi.org/10.1080/14756366.2017.1284068 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Akar, Zeynep Küçük, Murat Doğan, Hacer A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
title | A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
title_full | A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
title_fullStr | A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
title_full_unstemmed | A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
title_short | A new colorimetric DPPH(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
title_sort | new colorimetric dpph(•) scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009954/ https://www.ncbi.nlm.nih.gov/pubmed/28262029 http://dx.doi.org/10.1080/14756366.2017.1284068 |
work_keys_str_mv | AT akarzeynep anewcolorimetricdpphscavengingactivitymethodwithnoneedforaspectrophotometerappliedonsyntheticandnaturalantioxidantsandmedicinalherbs AT kucukmurat anewcolorimetricdpphscavengingactivitymethodwithnoneedforaspectrophotometerappliedonsyntheticandnaturalantioxidantsandmedicinalherbs AT doganhacer anewcolorimetricdpphscavengingactivitymethodwithnoneedforaspectrophotometerappliedonsyntheticandnaturalantioxidantsandmedicinalherbs AT akarzeynep newcolorimetricdpphscavengingactivitymethodwithnoneedforaspectrophotometerappliedonsyntheticandnaturalantioxidantsandmedicinalherbs AT kucukmurat newcolorimetricdpphscavengingactivitymethodwithnoneedforaspectrophotometerappliedonsyntheticandnaturalantioxidantsandmedicinalherbs AT doganhacer newcolorimetricdpphscavengingactivitymethodwithnoneedforaspectrophotometerappliedonsyntheticandnaturalantioxidantsandmedicinalherbs |