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Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes

Assembly and/or insertion of a subset of mitochondrial outer membrane (MOM) proteins, including subunits of the main MOM translocase, require the fungi-specific Mim1/Mim2 complex. So far it was unclear which proteins accomplish this task in other eukaryotes. Here, we show by reciprocal complementati...

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Autores principales: Vitali, Daniela G, Käser, Sandro, Kolb, Antonia, Dimmer, Kai S, Schneider, Andre, Rapaport, Doron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010339/
https://www.ncbi.nlm.nih.gov/pubmed/29923829
http://dx.doi.org/10.7554/eLife.34488
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author Vitali, Daniela G
Käser, Sandro
Kolb, Antonia
Dimmer, Kai S
Schneider, Andre
Rapaport, Doron
author_facet Vitali, Daniela G
Käser, Sandro
Kolb, Antonia
Dimmer, Kai S
Schneider, Andre
Rapaport, Doron
author_sort Vitali, Daniela G
collection PubMed
description Assembly and/or insertion of a subset of mitochondrial outer membrane (MOM) proteins, including subunits of the main MOM translocase, require the fungi-specific Mim1/Mim2 complex. So far it was unclear which proteins accomplish this task in other eukaryotes. Here, we show by reciprocal complementation that the MOM protein pATOM36 of trypanosomes is a functional analogue of yeast Mim1/Mim2 complex, even though these proteins show neither sequence nor topological similarity. Expression of pATOM36 rescues almost all growth, mitochondrial biogenesis, and morphology defects in yeast cells lacking Mim1 and/or Mim2. Conversely, co-expression of Mim1 and Mim2 restores the assembly and/or insertion defects of MOM proteins in trypanosomes ablated for pATOM36. Mim1/Mim2 and pATOM36 form native-like complexes when heterologously expressed, indicating that additional proteins are not part of these structures. Our findings indicate that Mim1/Mim2 and pATOM36 are the products of convergent evolution and arose only after the ancestors of fungi and trypanosomatids diverged.
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spelling pubmed-60103392018-06-21 Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes Vitali, Daniela G Käser, Sandro Kolb, Antonia Dimmer, Kai S Schneider, Andre Rapaport, Doron eLife Biochemistry and Chemical Biology Assembly and/or insertion of a subset of mitochondrial outer membrane (MOM) proteins, including subunits of the main MOM translocase, require the fungi-specific Mim1/Mim2 complex. So far it was unclear which proteins accomplish this task in other eukaryotes. Here, we show by reciprocal complementation that the MOM protein pATOM36 of trypanosomes is a functional analogue of yeast Mim1/Mim2 complex, even though these proteins show neither sequence nor topological similarity. Expression of pATOM36 rescues almost all growth, mitochondrial biogenesis, and morphology defects in yeast cells lacking Mim1 and/or Mim2. Conversely, co-expression of Mim1 and Mim2 restores the assembly and/or insertion defects of MOM proteins in trypanosomes ablated for pATOM36. Mim1/Mim2 and pATOM36 form native-like complexes when heterologously expressed, indicating that additional proteins are not part of these structures. Our findings indicate that Mim1/Mim2 and pATOM36 are the products of convergent evolution and arose only after the ancestors of fungi and trypanosomatids diverged. eLife Sciences Publications, Ltd 2018-06-20 /pmc/articles/PMC6010339/ /pubmed/29923829 http://dx.doi.org/10.7554/eLife.34488 Text en © 2018, Vitali et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Vitali, Daniela G
Käser, Sandro
Kolb, Antonia
Dimmer, Kai S
Schneider, Andre
Rapaport, Doron
Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
title Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
title_full Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
title_fullStr Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
title_full_unstemmed Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
title_short Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
title_sort independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010339/
https://www.ncbi.nlm.nih.gov/pubmed/29923829
http://dx.doi.org/10.7554/eLife.34488
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