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Transient AID expression for in situ mutagenesis with improved cellular fitness

Activation induced cytidine deaminase (AID) in germinal center B cells introduces somatic DNA mutations in transcribed immunoglobulin genes to increase antibody diversity. Ectopic expression of AID coupled with selection has been successfully employed to develop proteins with desirable properties. H...

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Detalles Bibliográficos
Autores principales:	Al-Qaisi, Talal Salem, Su, Yu-Cheng, Roffler, Steve R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010430/ https://www.ncbi.nlm.nih.gov/pubmed/29925928 http://dx.doi.org/10.1038/s41598-018-27717-2

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