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Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis

AIM: To investigate changes in gut microbiota and metabolism during nonalcoholic steatohepatitis (NASH) development in mice fed a methionine-choline-deficient (MCD) diet. METHODS: Twenty-four male C57BL/6J mice were equally divided into four groups and fed a methionine-choline-sufficient diet for 2...

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Autores principales: Ye, Jian-Zhong, Li, Ya-Ting, Wu, Wen-Rui, Shi, Ding, Fang, Dai-Qiong, Yang, Li-Ya, Bian, Xiao-Yuan, Wu, Jing-Jing, Wang, Qing, Jiang, Xian-Wan, Peng, Cong-Gao, Ye, Wan-Chun, Xia, Peng-Cheng, Li, Lan-Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010937/
https://www.ncbi.nlm.nih.gov/pubmed/29930468
http://dx.doi.org/10.3748/wjg.v24.i23.2468
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author Ye, Jian-Zhong
Li, Ya-Ting
Wu, Wen-Rui
Shi, Ding
Fang, Dai-Qiong
Yang, Li-Ya
Bian, Xiao-Yuan
Wu, Jing-Jing
Wang, Qing
Jiang, Xian-Wan
Peng, Cong-Gao
Ye, Wan-Chun
Xia, Peng-Cheng
Li, Lan-Juan
author_facet Ye, Jian-Zhong
Li, Ya-Ting
Wu, Wen-Rui
Shi, Ding
Fang, Dai-Qiong
Yang, Li-Ya
Bian, Xiao-Yuan
Wu, Jing-Jing
Wang, Qing
Jiang, Xian-Wan
Peng, Cong-Gao
Ye, Wan-Chun
Xia, Peng-Cheng
Li, Lan-Juan
author_sort Ye, Jian-Zhong
collection PubMed
description AIM: To investigate changes in gut microbiota and metabolism during nonalcoholic steatohepatitis (NASH) development in mice fed a methionine-choline-deficient (MCD) diet. METHODS: Twenty-four male C57BL/6J mice were equally divided into four groups and fed a methionine-choline-sufficient diet for 2 wk (Control 2w group, n = 6) or 4 wk (Control 4w group, n = 6) or the MCD diet for 2 wk (MCD 2w group, n = 6) or 4 wk (MCD 4w group, n = 6). Liver injury, fibrosis, and intestinal barrier function were evaluated after 2 and 4 wk of feeding. The fecal microbiome and metabolome were studied using 16s rRNA deep sequencing and gas chromatography-mass spectrometry. RESULTS: The mice fed the MCD diet presented with simple hepatic steatosis and slight intestinal barrier deterioration after 2 wk. After 4 wk of feeding with the MCD diet, however, the mice developed prominent NASH with liver fibrosis, and the intestinal barrier was more impaired. Compared with the control diet, the MCD diet induced gradual gut microbiota dysbiosis, as evidenced by a marked decrease in the abundance of Alistipes and the (Eubacterium) coprostanoligenes group (P < 0.001 and P < 0.05, respectively) and a significant increase in Ruminococcaceae UCG 014 abundance (P < 0.05) after 2 wk. At 4 wk, the MCD diet significantly reduced the promising probiotic Bifidobacterium levels and markedly promoted Bacteroides abundance (P < 0.05, and P < 0.01, respectively). The fecal metabolomic profile was also substantially altered by the MCD diet: At 2 wk, arachidic acid, hexadecane, palmitic acid, and tetracosane were selected as potential biomarkers that were significantly different in the corresponding control group, and at 4 wk, cholic acid, cholesterol, arachidic acid, tetracosane, and stearic acid were selected. CONCLUSION: The MCD diet induced persistent alterations in the gut microbiota and metabolome.
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spelling pubmed-60109372018-06-21 Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis Ye, Jian-Zhong Li, Ya-Ting Wu, Wen-Rui Shi, Ding Fang, Dai-Qiong Yang, Li-Ya Bian, Xiao-Yuan Wu, Jing-Jing Wang, Qing Jiang, Xian-Wan Peng, Cong-Gao Ye, Wan-Chun Xia, Peng-Cheng Li, Lan-Juan World J Gastroenterol Basic Study AIM: To investigate changes in gut microbiota and metabolism during nonalcoholic steatohepatitis (NASH) development in mice fed a methionine-choline-deficient (MCD) diet. METHODS: Twenty-four male C57BL/6J mice were equally divided into four groups and fed a methionine-choline-sufficient diet for 2 wk (Control 2w group, n = 6) or 4 wk (Control 4w group, n = 6) or the MCD diet for 2 wk (MCD 2w group, n = 6) or 4 wk (MCD 4w group, n = 6). Liver injury, fibrosis, and intestinal barrier function were evaluated after 2 and 4 wk of feeding. The fecal microbiome and metabolome were studied using 16s rRNA deep sequencing and gas chromatography-mass spectrometry. RESULTS: The mice fed the MCD diet presented with simple hepatic steatosis and slight intestinal barrier deterioration after 2 wk. After 4 wk of feeding with the MCD diet, however, the mice developed prominent NASH with liver fibrosis, and the intestinal barrier was more impaired. Compared with the control diet, the MCD diet induced gradual gut microbiota dysbiosis, as evidenced by a marked decrease in the abundance of Alistipes and the (Eubacterium) coprostanoligenes group (P < 0.001 and P < 0.05, respectively) and a significant increase in Ruminococcaceae UCG 014 abundance (P < 0.05) after 2 wk. At 4 wk, the MCD diet significantly reduced the promising probiotic Bifidobacterium levels and markedly promoted Bacteroides abundance (P < 0.05, and P < 0.01, respectively). The fecal metabolomic profile was also substantially altered by the MCD diet: At 2 wk, arachidic acid, hexadecane, palmitic acid, and tetracosane were selected as potential biomarkers that were significantly different in the corresponding control group, and at 4 wk, cholic acid, cholesterol, arachidic acid, tetracosane, and stearic acid were selected. CONCLUSION: The MCD diet induced persistent alterations in the gut microbiota and metabolome. Baishideng Publishing Group Inc 2018-06-21 2018-06-21 /pmc/articles/PMC6010937/ /pubmed/29930468 http://dx.doi.org/10.3748/wjg.v24.i23.2468 Text en ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Ye, Jian-Zhong
Li, Ya-Ting
Wu, Wen-Rui
Shi, Ding
Fang, Dai-Qiong
Yang, Li-Ya
Bian, Xiao-Yuan
Wu, Jing-Jing
Wang, Qing
Jiang, Xian-Wan
Peng, Cong-Gao
Ye, Wan-Chun
Xia, Peng-Cheng
Li, Lan-Juan
Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
title Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
title_full Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
title_fullStr Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
title_full_unstemmed Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
title_short Dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
title_sort dynamic alterations in the gut microbiota and metabolome during the development of methionine-choline-deficient diet-induced nonalcoholic steatohepatitis
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010937/
https://www.ncbi.nlm.nih.gov/pubmed/29930468
http://dx.doi.org/10.3748/wjg.v24.i23.2468
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