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MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1

BACKGROUND: MicroRNAs (miRs) have been implicated in the development and progression of osteosarcoma. Here, we aimed to illustrate the important role of miR-92a on the regulation of OS development which may help to establish a novel strategy for OS diagnosis and treatment. MATERIALS AND METHODS: Cel...

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Detalles Bibliográficos
Autores principales: Liu, Quanxiang, Song, Yang, Duan, Xianliang, Chang, Yuan, Guo, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011149/
https://www.ncbi.nlm.nih.gov/pubmed/29984257
http://dx.doi.org/10.1155/2018/9870693
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author Liu, Quanxiang
Song, Yang
Duan, Xianliang
Chang, Yuan
Guo, Jianping
author_facet Liu, Quanxiang
Song, Yang
Duan, Xianliang
Chang, Yuan
Guo, Jianping
author_sort Liu, Quanxiang
collection PubMed
description BACKGROUND: MicroRNAs (miRs) have been implicated in the development and progression of osteosarcoma. Here, we aimed to illustrate the important role of miR-92a on the regulation of OS development which may help to establish a novel strategy for OS diagnosis and treatment. MATERIALS AND METHODS: Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle and apoptosis were assessed by flow cytometry with PI and PI/Annexin-V stain, respectively. The expression of proteins was examined by western blot. qPCR was used to detect the expression of RNA. Cell migration was assayed with transwell assay. RESULTS: MiR-92a inhibited the proliferation and the migration of OS in vitro and reduced the volume of the tumour in vivo. Further, miR-92a enhanced cisplatin sensitivity of OS. MiR-92a directly targeted Notch1. CONCLUSION: Together, our results indicate that miR-92a inhibited cell growth, migration, and enhanced cisplatin sensitivity of OS cell by targeting Notch1.
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spelling pubmed-60111492018-07-08 MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1 Liu, Quanxiang Song, Yang Duan, Xianliang Chang, Yuan Guo, Jianping Biomed Res Int Research Article BACKGROUND: MicroRNAs (miRs) have been implicated in the development and progression of osteosarcoma. Here, we aimed to illustrate the important role of miR-92a on the regulation of OS development which may help to establish a novel strategy for OS diagnosis and treatment. MATERIALS AND METHODS: Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle and apoptosis were assessed by flow cytometry with PI and PI/Annexin-V stain, respectively. The expression of proteins was examined by western blot. qPCR was used to detect the expression of RNA. Cell migration was assayed with transwell assay. RESULTS: MiR-92a inhibited the proliferation and the migration of OS in vitro and reduced the volume of the tumour in vivo. Further, miR-92a enhanced cisplatin sensitivity of OS. MiR-92a directly targeted Notch1. CONCLUSION: Together, our results indicate that miR-92a inhibited cell growth, migration, and enhanced cisplatin sensitivity of OS cell by targeting Notch1. Hindawi 2018-06-07 /pmc/articles/PMC6011149/ /pubmed/29984257 http://dx.doi.org/10.1155/2018/9870693 Text en Copyright © 2018 Quanxiang Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Quanxiang
Song, Yang
Duan, Xianliang
Chang, Yuan
Guo, Jianping
MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
title MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
title_full MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
title_fullStr MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
title_full_unstemmed MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
title_short MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1
title_sort mir-92a inhibits the progress of osteosarcoma cells and increases the cisplatin sensitivity by targeting notch1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011149/
https://www.ncbi.nlm.nih.gov/pubmed/29984257
http://dx.doi.org/10.1155/2018/9870693
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