Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()

Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical result...

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Autores principales: Hardick, Justin, Metzgar, David, Risen, Lisa, Myers, Christopher, Balansay, Melinda, Malcom, Trent, Rothman, Richard, Gaydos, Charlotte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013072/
https://www.ncbi.nlm.nih.gov/pubmed/29550057
http://dx.doi.org/10.1016/j.diagmicrobio.2018.02.011
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author Hardick, Justin
Metzgar, David
Risen, Lisa
Myers, Christopher
Balansay, Melinda
Malcom, Trent
Rothman, Richard
Gaydos, Charlotte
author_facet Hardick, Justin
Metzgar, David
Risen, Lisa
Myers, Christopher
Balansay, Melinda
Malcom, Trent
Rothman, Richard
Gaydos, Charlotte
author_sort Hardick, Justin
collection PubMed
description Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the MAP was 30 copies/assay for RSV and 1500 copies/assay for Middle East respiratory syndrome coronavirus.
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spelling pubmed-60130722018-07-01 Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()() Hardick, Justin Metzgar, David Risen, Lisa Myers, Christopher Balansay, Melinda Malcom, Trent Rothman, Richard Gaydos, Charlotte Diagn Microbiol Infect Dis Article Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the MAP was 30 copies/assay for RSV and 1500 copies/assay for Middle East respiratory syndrome coronavirus. Elsevier Inc. 2018-07 2018-02-17 /pmc/articles/PMC6013072/ /pubmed/29550057 http://dx.doi.org/10.1016/j.diagmicrobio.2018.02.011 Text en © 2018 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hardick, Justin
Metzgar, David
Risen, Lisa
Myers, Christopher
Balansay, Melinda
Malcom, Trent
Rothman, Richard
Gaydos, Charlotte
Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()
title Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()
title_full Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()
title_fullStr Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()
title_full_unstemmed Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()
title_short Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus()()
title_sort initial performance evaluation of a spotted array mobile analysis platform (map) for the detection of influenza a/b, rsv, and mers coronavirus()()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013072/
https://www.ncbi.nlm.nih.gov/pubmed/29550057
http://dx.doi.org/10.1016/j.diagmicrobio.2018.02.011
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