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Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis

Reactive oxygen species (ROS) are molecules that play a prominent role in plant response to numerous stresses, including plant interactions with herbivores. Previous findings indicate that Arabidopsis plants showed an increase in H(2)O(2) accumulation after Tetranychus urticae infestation. Despite i...

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Autores principales: Santamaría, M. Estrella, Arnaiz, Ana, Velasco-Arroyo, Blanca, Grbic, Vojislava, Diaz, Isabel, Martinez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013483/
https://www.ncbi.nlm.nih.gov/pubmed/29930298
http://dx.doi.org/10.1038/s41598-018-27904-1
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author Santamaría, M. Estrella
Arnaiz, Ana
Velasco-Arroyo, Blanca
Grbic, Vojislava
Diaz, Isabel
Martinez, Manuel
author_facet Santamaría, M. Estrella
Arnaiz, Ana
Velasco-Arroyo, Blanca
Grbic, Vojislava
Diaz, Isabel
Martinez, Manuel
author_sort Santamaría, M. Estrella
collection PubMed
description Reactive oxygen species (ROS) are molecules that play a prominent role in plant response to numerous stresses, including plant interactions with herbivores. Previous findings indicate that Arabidopsis plants showed an increase in H(2)O(2) accumulation after Tetranychus urticae infestation. Despite its importance, no information has been reported on the relationships between ROS-metabolizing systems and the spider mite-triggered plant-induced responses. In this work, four ROS-related genes that were differentially expressed between the resistant Bla-2 and the susceptible Kon Arabidopsis accessions were selected for the analysis. These genes encode proteins putatively involved in the generation (BBE22) and degradation (GPX7 and GSTU4) of H(2)O(2), and in the degradation of ascorbate (AO). Overexpressing BBE22 and silencing GPX7, GSTU4 and AO resulted in higher leaf damage and better mite performance relative to the wild-type plants. Minor effects on H(2)O(2) accumulation obscure major effects on the expression of genes related to ROS-metabolism and JA and SA signaling pathways, and on ROS-related enzymatic activities. In conclusion, the integration of ROS and ROS-related compounds and enzymes in the response of Arabidopsis to the spider mite T. urticae was confirmed. However, the complex network involved in ROS signaling makes difficult to predict the impact of a specific genetic manipulation.
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spelling pubmed-60134832018-06-27 Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis Santamaría, M. Estrella Arnaiz, Ana Velasco-Arroyo, Blanca Grbic, Vojislava Diaz, Isabel Martinez, Manuel Sci Rep Article Reactive oxygen species (ROS) are molecules that play a prominent role in plant response to numerous stresses, including plant interactions with herbivores. Previous findings indicate that Arabidopsis plants showed an increase in H(2)O(2) accumulation after Tetranychus urticae infestation. Despite its importance, no information has been reported on the relationships between ROS-metabolizing systems and the spider mite-triggered plant-induced responses. In this work, four ROS-related genes that were differentially expressed between the resistant Bla-2 and the susceptible Kon Arabidopsis accessions were selected for the analysis. These genes encode proteins putatively involved in the generation (BBE22) and degradation (GPX7 and GSTU4) of H(2)O(2), and in the degradation of ascorbate (AO). Overexpressing BBE22 and silencing GPX7, GSTU4 and AO resulted in higher leaf damage and better mite performance relative to the wild-type plants. Minor effects on H(2)O(2) accumulation obscure major effects on the expression of genes related to ROS-metabolism and JA and SA signaling pathways, and on ROS-related enzymatic activities. In conclusion, the integration of ROS and ROS-related compounds and enzymes in the response of Arabidopsis to the spider mite T. urticae was confirmed. However, the complex network involved in ROS signaling makes difficult to predict the impact of a specific genetic manipulation. Nature Publishing Group UK 2018-06-21 /pmc/articles/PMC6013483/ /pubmed/29930298 http://dx.doi.org/10.1038/s41598-018-27904-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Santamaría, M. Estrella
Arnaiz, Ana
Velasco-Arroyo, Blanca
Grbic, Vojislava
Diaz, Isabel
Martinez, Manuel
Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
title Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
title_full Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
title_fullStr Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
title_full_unstemmed Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
title_short Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
title_sort arabidopsis response to the spider mite tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013483/
https://www.ncbi.nlm.nih.gov/pubmed/29930298
http://dx.doi.org/10.1038/s41598-018-27904-1
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