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On the current role of hydratases in biocatalysis
Water addition to carbon-carbon double bonds provides access to value-added products from inexpensive organic feedstock. This interesting but relatively little-studied reaction is catalysed by hydratases in a highly regio- and enantiospecific fashion with excellent atom economy. Considering that asy...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013536/ https://www.ncbi.nlm.nih.gov/pubmed/29785499 http://dx.doi.org/10.1007/s00253-018-9065-7 |
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author | Engleder, Matthias Pichler, Harald |
author_facet | Engleder, Matthias Pichler, Harald |
author_sort | Engleder, Matthias |
collection | PubMed |
description | Water addition to carbon-carbon double bonds provides access to value-added products from inexpensive organic feedstock. This interesting but relatively little-studied reaction is catalysed by hydratases in a highly regio- and enantiospecific fashion with excellent atom economy. Considering that asymmetric hydration of (non-activated) carbon-carbon double bonds is virtually impossible with current organic chemistry, enzymatic hydration reactions are highly attractive for industrial applications. Hydratases have been known for several decades but their biocatalytic potential has only been explored over the past 15 years. As a result, a considerable amount of information on this enzyme group has become available, enabling their development for practical applications. This review focuses on hydratases catalysing water addition to non-activated carbon-carbon double bonds, and examines hydratases from a biochemical, structural and mechanistic angle. Current challenges and opportunities in hydration biocatalysis are discussed, and, ultimately, their potential for organic synthesis is highlighted. |
format | Online Article Text |
id | pubmed-6013536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-60135362018-06-25 On the current role of hydratases in biocatalysis Engleder, Matthias Pichler, Harald Appl Microbiol Biotechnol Mini-Review Water addition to carbon-carbon double bonds provides access to value-added products from inexpensive organic feedstock. This interesting but relatively little-studied reaction is catalysed by hydratases in a highly regio- and enantiospecific fashion with excellent atom economy. Considering that asymmetric hydration of (non-activated) carbon-carbon double bonds is virtually impossible with current organic chemistry, enzymatic hydration reactions are highly attractive for industrial applications. Hydratases have been known for several decades but their biocatalytic potential has only been explored over the past 15 years. As a result, a considerable amount of information on this enzyme group has become available, enabling their development for practical applications. This review focuses on hydratases catalysing water addition to non-activated carbon-carbon double bonds, and examines hydratases from a biochemical, structural and mechanistic angle. Current challenges and opportunities in hydration biocatalysis are discussed, and, ultimately, their potential for organic synthesis is highlighted. Springer Berlin Heidelberg 2018-05-21 2018 /pmc/articles/PMC6013536/ /pubmed/29785499 http://dx.doi.org/10.1007/s00253-018-9065-7 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Mini-Review Engleder, Matthias Pichler, Harald On the current role of hydratases in biocatalysis |
title | On the current role of hydratases in biocatalysis |
title_full | On the current role of hydratases in biocatalysis |
title_fullStr | On the current role of hydratases in biocatalysis |
title_full_unstemmed | On the current role of hydratases in biocatalysis |
title_short | On the current role of hydratases in biocatalysis |
title_sort | on the current role of hydratases in biocatalysis |
topic | Mini-Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013536/ https://www.ncbi.nlm.nih.gov/pubmed/29785499 http://dx.doi.org/10.1007/s00253-018-9065-7 |
work_keys_str_mv | AT engledermatthias onthecurrentroleofhydratasesinbiocatalysis AT pichlerharald onthecurrentroleofhydratasesinbiocatalysis |