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OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds

Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and fi...

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Autores principales: Bian, Wenxin, Meng, Buliang, Li, Xiaojie, Wang, Siyuan, Cao, Xiaoqing, Liu, Naixin, Yang, Meifeng, Tang, Jing, Wang, Ying, Yang, Xinwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013704/
https://www.ncbi.nlm.nih.gov/pubmed/29752337
http://dx.doi.org/10.1042/BSR20180215
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author Bian, Wenxin
Meng, Buliang
Li, Xiaojie
Wang, Siyuan
Cao, Xiaoqing
Liu, Naixin
Yang, Meifeng
Tang, Jing
Wang, Ying
Yang, Xinwang
author_facet Bian, Wenxin
Meng, Buliang
Li, Xiaojie
Wang, Siyuan
Cao, Xiaoqing
Liu, Naixin
Yang, Meifeng
Tang, Jing
Wang, Ying
Yang, Xinwang
author_sort Bian, Wenxin
collection PubMed
description Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and financially. Therefore, it is urgent to develop new drugs to accelerate the healing of wounds. Bioactive peptides, which are relatively low cost, easy to produce, store and transport, have become an excellent choice. In this research, we identified a novel peptide OA-GL21, with an amino acid sequence of ‘GLLSGHYGRVVSTQSGHYGRG’, from the skin secretions of Odorrana andersonii. Our results showed that OA-GL21 exerted the ability to promote wound healing of human keratinocytes (HaCaT) and human fibroblasts in a dose- and time-denpendent manner. However, OA-GL21 had no significant effect on the proliferation of these two cells. Significantly, OA-GL21 showed obvious ability to promote wound healing in the full-thickness skin wound model in dose- and scar-free manners. Further studies showed that OA-GL21 had no direct antibacterial, hemolytic, and acute toxic activity; it had weak antioxidant activities but high stability. In conclusion, this research proved the promoting effects of OA-GL21 on cellular and animal wounds, and thus provided a new peptide template for the development of wound-repairing drugs.
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spelling pubmed-60137042018-07-06 OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds Bian, Wenxin Meng, Buliang Li, Xiaojie Wang, Siyuan Cao, Xiaoqing Liu, Naixin Yang, Meifeng Tang, Jing Wang, Ying Yang, Xinwang Biosci Rep Research Articles Nowadays, the number of chronic trauma cases caused by a variety of factors such as the world’s population-ageing and chronic diseases is increasing steadily, and thus effective treatment for chronic wounds has become a severe clinical challenge, which also burdens the patient both physically and financially. Therefore, it is urgent to develop new drugs to accelerate the healing of wounds. Bioactive peptides, which are relatively low cost, easy to produce, store and transport, have become an excellent choice. In this research, we identified a novel peptide OA-GL21, with an amino acid sequence of ‘GLLSGHYGRVVSTQSGHYGRG’, from the skin secretions of Odorrana andersonii. Our results showed that OA-GL21 exerted the ability to promote wound healing of human keratinocytes (HaCaT) and human fibroblasts in a dose- and time-denpendent manner. However, OA-GL21 had no significant effect on the proliferation of these two cells. Significantly, OA-GL21 showed obvious ability to promote wound healing in the full-thickness skin wound model in dose- and scar-free manners. Further studies showed that OA-GL21 had no direct antibacterial, hemolytic, and acute toxic activity; it had weak antioxidant activities but high stability. In conclusion, this research proved the promoting effects of OA-GL21 on cellular and animal wounds, and thus provided a new peptide template for the development of wound-repairing drugs. Portland Press Ltd. 2018-06-21 /pmc/articles/PMC6013704/ /pubmed/29752337 http://dx.doi.org/10.1042/BSR20180215 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Bian, Wenxin
Meng, Buliang
Li, Xiaojie
Wang, Siyuan
Cao, Xiaoqing
Liu, Naixin
Yang, Meifeng
Tang, Jing
Wang, Ying
Yang, Xinwang
OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds
title OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds
title_full OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds
title_fullStr OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds
title_full_unstemmed OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds
title_short OA-GL21, a novel bioactive peptide from Odorrana andersonii, accelerated the healing of skin wounds
title_sort oa-gl21, a novel bioactive peptide from odorrana andersonii, accelerated the healing of skin wounds
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013704/
https://www.ncbi.nlm.nih.gov/pubmed/29752337
http://dx.doi.org/10.1042/BSR20180215
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