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MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma

Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade, and poor prognosis. Down-regulati...

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Detalles Bibliográficos
Autores principales: Wang, Dan, Wang, Hongyan, Li, Yichun, Li, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013705/
https://www.ncbi.nlm.nih.gov/pubmed/29871972
http://dx.doi.org/10.1042/BSR20180668
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author Wang, Dan
Wang, Hongyan
Li, Yichun
Li, Qian
author_facet Wang, Dan
Wang, Hongyan
Li, Yichun
Li, Qian
author_sort Wang, Dan
collection PubMed
description Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade, and poor prognosis. Down-regulation of MCM5 inhibited cervical adenocarcinoma cell proliferation. The purpose of the present study is to search and confirm valuable microRNAs (miRNAs), which target MCM5 to modulate cervical adenocarcinoma cell proliferation. In our results, we found that levels of miR-362-3p expression were reduced in cervical adenocarcinoma tissues and cell lines. Moreover, 3′-UTR of MCM5 had binding site of miR-362-3p through analyzing Targetscan database and miRanda database, and there were an inverse association between miR-362-3p and MCM5 in cervical adenocarcinoma tissues. Furthermore, we verified miR-362-3p directly targeted to 3′-UTR of DCLK1 by luciferase reporter assay, and negatively regulated mRNA and protein expressions of MCM5 by qPCR and Western blot. Then, we conducted gain-of-function study and rescued-function study, and found that miR-362-3p served as a tumor suppressive miRNA to modulate cervical adenocarcinoma cell proliferation through regulating the functional target MCM5. Finally, we analyzed correlations between miR-362-3p expression and clinicopathological characteristics and observed that miR-362-3p low expression was associated with advanced clinical stage and poor prognosis. In conclusion, miR-362-3p is a tumor suppressive miRNA in cervical adenocarcinoma.
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spelling pubmed-60137052018-07-06 MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma Wang, Dan Wang, Hongyan Li, Yichun Li, Qian Biosci Rep Research Articles Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade, and poor prognosis. Down-regulation of MCM5 inhibited cervical adenocarcinoma cell proliferation. The purpose of the present study is to search and confirm valuable microRNAs (miRNAs), which target MCM5 to modulate cervical adenocarcinoma cell proliferation. In our results, we found that levels of miR-362-3p expression were reduced in cervical adenocarcinoma tissues and cell lines. Moreover, 3′-UTR of MCM5 had binding site of miR-362-3p through analyzing Targetscan database and miRanda database, and there were an inverse association between miR-362-3p and MCM5 in cervical adenocarcinoma tissues. Furthermore, we verified miR-362-3p directly targeted to 3′-UTR of DCLK1 by luciferase reporter assay, and negatively regulated mRNA and protein expressions of MCM5 by qPCR and Western blot. Then, we conducted gain-of-function study and rescued-function study, and found that miR-362-3p served as a tumor suppressive miRNA to modulate cervical adenocarcinoma cell proliferation through regulating the functional target MCM5. Finally, we analyzed correlations between miR-362-3p expression and clinicopathological characteristics and observed that miR-362-3p low expression was associated with advanced clinical stage and poor prognosis. In conclusion, miR-362-3p is a tumor suppressive miRNA in cervical adenocarcinoma. Portland Press Ltd. 2018-06-21 /pmc/articles/PMC6013705/ /pubmed/29871972 http://dx.doi.org/10.1042/BSR20180668 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Wang, Dan
Wang, Hongyan
Li, Yichun
Li, Qian
MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
title MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
title_full MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
title_fullStr MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
title_full_unstemmed MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
title_short MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
title_sort mir-362-3p functions as a tumor suppressor through targeting mcm5 in cervical adenocarcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013705/
https://www.ncbi.nlm.nih.gov/pubmed/29871972
http://dx.doi.org/10.1042/BSR20180668
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