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MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma
Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade, and poor prognosis. Down-regulati...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013705/ https://www.ncbi.nlm.nih.gov/pubmed/29871972 http://dx.doi.org/10.1042/BSR20180668 |
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author | Wang, Dan Wang, Hongyan Li, Yichun Li, Qian |
author_facet | Wang, Dan Wang, Hongyan Li, Yichun Li, Qian |
author_sort | Wang, Dan |
collection | PubMed |
description | Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade, and poor prognosis. Down-regulation of MCM5 inhibited cervical adenocarcinoma cell proliferation. The purpose of the present study is to search and confirm valuable microRNAs (miRNAs), which target MCM5 to modulate cervical adenocarcinoma cell proliferation. In our results, we found that levels of miR-362-3p expression were reduced in cervical adenocarcinoma tissues and cell lines. Moreover, 3′-UTR of MCM5 had binding site of miR-362-3p through analyzing Targetscan database and miRanda database, and there were an inverse association between miR-362-3p and MCM5 in cervical adenocarcinoma tissues. Furthermore, we verified miR-362-3p directly targeted to 3′-UTR of DCLK1 by luciferase reporter assay, and negatively regulated mRNA and protein expressions of MCM5 by qPCR and Western blot. Then, we conducted gain-of-function study and rescued-function study, and found that miR-362-3p served as a tumor suppressive miRNA to modulate cervical adenocarcinoma cell proliferation through regulating the functional target MCM5. Finally, we analyzed correlations between miR-362-3p expression and clinicopathological characteristics and observed that miR-362-3p low expression was associated with advanced clinical stage and poor prognosis. In conclusion, miR-362-3p is a tumor suppressive miRNA in cervical adenocarcinoma. |
format | Online Article Text |
id | pubmed-6013705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60137052018-07-06 MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma Wang, Dan Wang, Hongyan Li, Yichun Li, Qian Biosci Rep Research Articles Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade, and poor prognosis. Down-regulation of MCM5 inhibited cervical adenocarcinoma cell proliferation. The purpose of the present study is to search and confirm valuable microRNAs (miRNAs), which target MCM5 to modulate cervical adenocarcinoma cell proliferation. In our results, we found that levels of miR-362-3p expression were reduced in cervical adenocarcinoma tissues and cell lines. Moreover, 3′-UTR of MCM5 had binding site of miR-362-3p through analyzing Targetscan database and miRanda database, and there were an inverse association between miR-362-3p and MCM5 in cervical adenocarcinoma tissues. Furthermore, we verified miR-362-3p directly targeted to 3′-UTR of DCLK1 by luciferase reporter assay, and negatively regulated mRNA and protein expressions of MCM5 by qPCR and Western blot. Then, we conducted gain-of-function study and rescued-function study, and found that miR-362-3p served as a tumor suppressive miRNA to modulate cervical adenocarcinoma cell proliferation through regulating the functional target MCM5. Finally, we analyzed correlations between miR-362-3p expression and clinicopathological characteristics and observed that miR-362-3p low expression was associated with advanced clinical stage and poor prognosis. In conclusion, miR-362-3p is a tumor suppressive miRNA in cervical adenocarcinoma. Portland Press Ltd. 2018-06-21 /pmc/articles/PMC6013705/ /pubmed/29871972 http://dx.doi.org/10.1042/BSR20180668 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Wang, Dan Wang, Hongyan Li, Yichun Li, Qian MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma |
title | MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma |
title_full | MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma |
title_fullStr | MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma |
title_full_unstemmed | MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma |
title_short | MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma |
title_sort | mir-362-3p functions as a tumor suppressor through targeting mcm5 in cervical adenocarcinoma |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013705/ https://www.ncbi.nlm.nih.gov/pubmed/29871972 http://dx.doi.org/10.1042/BSR20180668 |
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