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Mps1 promotes chromosome meiotic chromosome biorientation through Dam1

In budding yeast meiosis, homologous chromosomes become linked by chiasmata and then move back and forth on the spindle until they are bioriented, with the kinetochores of the partners attached to microtubules from opposite spindle poles. Certain mutations in the conserved kinase, Mps1, result in ca...

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Detalles Bibliográficos
Autores principales: Meyer, Régis E., Brown, Jamin, Beck, Lindsay, Dawson, Dean S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014172/
https://www.ncbi.nlm.nih.gov/pubmed/29237818
http://dx.doi.org/10.1091/mbc.E17-08-0503
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author Meyer, Régis E.
Brown, Jamin
Beck, Lindsay
Dawson, Dean S.
author_facet Meyer, Régis E.
Brown, Jamin
Beck, Lindsay
Dawson, Dean S.
author_sort Meyer, Régis E.
collection PubMed
description In budding yeast meiosis, homologous chromosomes become linked by chiasmata and then move back and forth on the spindle until they are bioriented, with the kinetochores of the partners attached to microtubules from opposite spindle poles. Certain mutations in the conserved kinase, Mps1, result in catastrophic meiotic segregation errors but mild mitotic defects. We tested whether Dam1, a known substrate of Mps1, was necessary for its critical meiotic role. We found that kinetochore–microtubule attachments are established even when Dam1 is not phosphorylated by Mps1, but that Mps1 phosphorylation of Dam1 sustains those connections. But the meiotic defects when Dam1 is not phosphorylated are not nearly as catastrophic as when Mps1 is inactivated. The results demonstrate that one meiotic role of Mps1 is to stabilize connections that have been established between kinetochores and microtubles by phosphorylating Dam1.
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spelling pubmed-60141722018-06-22 Mps1 promotes chromosome meiotic chromosome biorientation through Dam1 Meyer, Régis E. Brown, Jamin Beck, Lindsay Dawson, Dean S. Mol Biol Cell Articles In budding yeast meiosis, homologous chromosomes become linked by chiasmata and then move back and forth on the spindle until they are bioriented, with the kinetochores of the partners attached to microtubules from opposite spindle poles. Certain mutations in the conserved kinase, Mps1, result in catastrophic meiotic segregation errors but mild mitotic defects. We tested whether Dam1, a known substrate of Mps1, was necessary for its critical meiotic role. We found that kinetochore–microtubule attachments are established even when Dam1 is not phosphorylated by Mps1, but that Mps1 phosphorylation of Dam1 sustains those connections. But the meiotic defects when Dam1 is not phosphorylated are not nearly as catastrophic as when Mps1 is inactivated. The results demonstrate that one meiotic role of Mps1 is to stabilize connections that have been established between kinetochores and microtubles by phosphorylating Dam1. The American Society for Cell Biology 2018-02-15 /pmc/articles/PMC6014172/ /pubmed/29237818 http://dx.doi.org/10.1091/mbc.E17-08-0503 Text en © 2018 Meyer et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Meyer, Régis E.
Brown, Jamin
Beck, Lindsay
Dawson, Dean S.
Mps1 promotes chromosome meiotic chromosome biorientation through Dam1
title Mps1 promotes chromosome meiotic chromosome biorientation through Dam1
title_full Mps1 promotes chromosome meiotic chromosome biorientation through Dam1
title_fullStr Mps1 promotes chromosome meiotic chromosome biorientation through Dam1
title_full_unstemmed Mps1 promotes chromosome meiotic chromosome biorientation through Dam1
title_short Mps1 promotes chromosome meiotic chromosome biorientation through Dam1
title_sort mps1 promotes chromosome meiotic chromosome biorientation through dam1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014172/
https://www.ncbi.nlm.nih.gov/pubmed/29237818
http://dx.doi.org/10.1091/mbc.E17-08-0503
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