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Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks
This study presents the morphological and physiological characterization of the salivary glands of semi-engorged Amblyomma triste females. Unfed individuals were placed on New Zealand White rabbits for feeding and the females, after 4 days, were collected, dissected and the salivary glands were subm...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014189/ https://www.ncbi.nlm.nih.gov/pubmed/30023188 http://dx.doi.org/10.1016/j.jmau.2014.10.003 |
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author | de Oliveira, Patrícia Rosa Scopinho Furquim, Karim Christina Bechara, Gervásio Henrique Camargo-Mathias, Maria Izabel |
author_facet | de Oliveira, Patrícia Rosa Scopinho Furquim, Karim Christina Bechara, Gervásio Henrique Camargo-Mathias, Maria Izabel |
author_sort | de Oliveira, Patrícia Rosa |
collection | PubMed |
description | This study presents the morphological and physiological characterization of the salivary glands of semi-engorged Amblyomma triste females. Unfed individuals were placed on New Zealand White rabbits for feeding and the females, after 4 days, were collected, dissected and the salivary glands were submitted to the application of histological (hematoxylin–eosin technique) and histochemical tests for the detection of protein (bromophenol blue technique, polysaccharides (periodic acid–Schiff technique), lipid (Nile blue technique) and calcium (von Kossa technique). The histological results show that the glandular tissue is composed by a system of ducts and three types of acini (I, II and III). The acini I are formed by a large central cell surrounded by several smaller agranular peripheral cells. Acini II are formed by cells a, c1, c2, c3 and c5, which are full of secretion granules. Acini III are constituted by cells d, e and f; the former two contain secretion granules, the latter is agranular. The glandular histochemical composition was also verified. Data obtained here will certainly help in the understanding of the cellular morphology and of the general physiology of these organs in this specie, providing important information for the creation of scientific bases which will contribute for the development of more specific and efficient methods of control. |
format | Online Article Text |
id | pubmed-6014189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60141892018-07-18 Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks de Oliveira, Patrícia Rosa Scopinho Furquim, Karim Christina Bechara, Gervásio Henrique Camargo-Mathias, Maria Izabel J Microsc Ultrastruct Original Article This study presents the morphological and physiological characterization of the salivary glands of semi-engorged Amblyomma triste females. Unfed individuals were placed on New Zealand White rabbits for feeding and the females, after 4 days, were collected, dissected and the salivary glands were submitted to the application of histological (hematoxylin–eosin technique) and histochemical tests for the detection of protein (bromophenol blue technique, polysaccharides (periodic acid–Schiff technique), lipid (Nile blue technique) and calcium (von Kossa technique). The histological results show that the glandular tissue is composed by a system of ducts and three types of acini (I, II and III). The acini I are formed by a large central cell surrounded by several smaller agranular peripheral cells. Acini II are formed by cells a, c1, c2, c3 and c5, which are full of secretion granules. Acini III are constituted by cells d, e and f; the former two contain secretion granules, the latter is agranular. The glandular histochemical composition was also verified. Data obtained here will certainly help in the understanding of the cellular morphology and of the general physiology of these organs in this specie, providing important information for the creation of scientific bases which will contribute for the development of more specific and efficient methods of control. Medknow Publications & Media Pvt Ltd 2015 2014-10-17 /pmc/articles/PMC6014189/ /pubmed/30023188 http://dx.doi.org/10.1016/j.jmau.2014.10.003 Text en Copyright: © 2014 Saudi Society of Microscopes http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article de Oliveira, Patrícia Rosa Scopinho Furquim, Karim Christina Bechara, Gervásio Henrique Camargo-Mathias, Maria Izabel Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks |
title | Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks |
title_full | Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks |
title_fullStr | Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks |
title_full_unstemmed | Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks |
title_short | Morpho-histochemical characterization of the salivary glands of semi-engorged Amblyomma triste (Koch, 1844) (Acari: Ixodidae) female ticks |
title_sort | morpho-histochemical characterization of the salivary glands of semi-engorged amblyomma triste (koch, 1844) (acari: ixodidae) female ticks |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014189/ https://www.ncbi.nlm.nih.gov/pubmed/30023188 http://dx.doi.org/10.1016/j.jmau.2014.10.003 |
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