Cargando…

A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy

Drosophila antennae is gaining attention to study the hearing molecules and its mechanism in last few decades. Various molecules required for the formation of hearing organ is conserved between Drosophila and human being. This suggests Drosophila can be used as a model organism to decipher the verte...

Descripción completa

Detalles Bibliográficos
Autor principal: Mishra, Monalisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014221/
https://www.ncbi.nlm.nih.gov/pubmed/30023175
http://dx.doi.org/10.1016/j.jmau.2014.06.004
_version_ 1783334190455980032
author Mishra, Monalisa
author_facet Mishra, Monalisa
author_sort Mishra, Monalisa
collection PubMed
description Drosophila antennae is gaining attention to study the hearing molecules and its mechanism in last few decades. Various molecules required for the formation of hearing organ is conserved between Drosophila and human being. This suggests Drosophila can be used as a model organism to decipher the vertebrate hearing mechanism. In this context a protocol describing the fixation, sectioning and staining of antennae is lacking from the literature. The current paper describes various commercially available markers of the antennae to visualise it under confocal microscope.
format Online
Article
Text
id pubmed-6014221
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-60142212018-07-18 A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy Mishra, Monalisa J Microsc Ultrastruct Review Article Drosophila antennae is gaining attention to study the hearing molecules and its mechanism in last few decades. Various molecules required for the formation of hearing organ is conserved between Drosophila and human being. This suggests Drosophila can be used as a model organism to decipher the vertebrate hearing mechanism. In this context a protocol describing the fixation, sectioning and staining of antennae is lacking from the literature. The current paper describes various commercially available markers of the antennae to visualise it under confocal microscope. Medknow Publications & Media Pvt Ltd 2015 2014-07-11 /pmc/articles/PMC6014221/ /pubmed/30023175 http://dx.doi.org/10.1016/j.jmau.2014.06.004 Text en Copyright: © 2014 Saudi Society of Microscopes http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Mishra, Monalisa
A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
title A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
title_full A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
title_fullStr A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
title_full_unstemmed A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
title_short A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
title_sort quick method to investigate the drosophila johnston’s organ by confocal microscopy
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014221/
https://www.ncbi.nlm.nih.gov/pubmed/30023175
http://dx.doi.org/10.1016/j.jmau.2014.06.004
work_keys_str_mv AT mishramonalisa aquickmethodtoinvestigatethedrosophilajohnstonsorganbyconfocalmicroscopy
AT mishramonalisa quickmethodtoinvestigatethedrosophilajohnstonsorganbyconfocalmicroscopy