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A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy
Drosophila antennae is gaining attention to study the hearing molecules and its mechanism in last few decades. Various molecules required for the formation of hearing organ is conserved between Drosophila and human being. This suggests Drosophila can be used as a model organism to decipher the verte...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014221/ https://www.ncbi.nlm.nih.gov/pubmed/30023175 http://dx.doi.org/10.1016/j.jmau.2014.06.004 |
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author | Mishra, Monalisa |
author_facet | Mishra, Monalisa |
author_sort | Mishra, Monalisa |
collection | PubMed |
description | Drosophila antennae is gaining attention to study the hearing molecules and its mechanism in last few decades. Various molecules required for the formation of hearing organ is conserved between Drosophila and human being. This suggests Drosophila can be used as a model organism to decipher the vertebrate hearing mechanism. In this context a protocol describing the fixation, sectioning and staining of antennae is lacking from the literature. The current paper describes various commercially available markers of the antennae to visualise it under confocal microscope. |
format | Online Article Text |
id | pubmed-6014221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60142212018-07-18 A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy Mishra, Monalisa J Microsc Ultrastruct Review Article Drosophila antennae is gaining attention to study the hearing molecules and its mechanism in last few decades. Various molecules required for the formation of hearing organ is conserved between Drosophila and human being. This suggests Drosophila can be used as a model organism to decipher the vertebrate hearing mechanism. In this context a protocol describing the fixation, sectioning and staining of antennae is lacking from the literature. The current paper describes various commercially available markers of the antennae to visualise it under confocal microscope. Medknow Publications & Media Pvt Ltd 2015 2014-07-11 /pmc/articles/PMC6014221/ /pubmed/30023175 http://dx.doi.org/10.1016/j.jmau.2014.06.004 Text en Copyright: © 2014 Saudi Society of Microscopes http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Review Article Mishra, Monalisa A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy |
title | A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy |
title_full | A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy |
title_fullStr | A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy |
title_full_unstemmed | A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy |
title_short | A quick method to investigate the Drosophila Johnston’s organ by confocal microscopy |
title_sort | quick method to investigate the drosophila johnston’s organ by confocal microscopy |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014221/ https://www.ncbi.nlm.nih.gov/pubmed/30023175 http://dx.doi.org/10.1016/j.jmau.2014.06.004 |
work_keys_str_mv | AT mishramonalisa aquickmethodtoinvestigatethedrosophilajohnstonsorganbyconfocalmicroscopy AT mishramonalisa quickmethodtoinvestigatethedrosophilajohnstonsorganbyconfocalmicroscopy |