Cargando…

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Micropropagation via induction, multiplication and development of nodular cultures (NCs) is an efficient regeneration system for Bromeliaceae, a family of endangered monocot plants with ornamental value. Therefore, the present work aimed to induce NCs from seeds and leaf explants of Vriesea in order...

Descripción completa

Detalles Bibliográficos
Autores principales: Corredor-Prado, Jenny P., Schmidt, Eder C., Guerra, Miguel P., Bouzon, Zenilda L., Dal Vesco, Lírio L., Pescador, Rosete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014276/
https://www.ncbi.nlm.nih.gov/pubmed/30023200
http://dx.doi.org/10.1016/j.jmau.2015.04.001
_version_ 1783334203272724480
author Corredor-Prado, Jenny P.
Schmidt, Eder C.
Guerra, Miguel P.
Bouzon, Zenilda L.
Dal Vesco, Lírio L.
Pescador, Rosete
author_facet Corredor-Prado, Jenny P.
Schmidt, Eder C.
Guerra, Miguel P.
Bouzon, Zenilda L.
Dal Vesco, Lírio L.
Pescador, Rosete
author_sort Corredor-Prado, Jenny P.
collection PubMed
description Micropropagation via induction, multiplication and development of nodular cultures (NCs) is an efficient regeneration system for Bromeliaceae, a family of endangered monocot plants with ornamental value. Therefore, the present work aimed to induce NCs from seeds and leaf explants of Vriesea in order to characterize the morphological and histochemical aspects of induction and formation of these cultures. Seeds of Vriesea friburgensis var. paludosa were sterilized and inoculated into liquid culture media supplemented with different concentrations and combinations of growth regulators. Leaf explants of Vriesea reitzii were inoculated into medium supplemented with 4 μM α-naphthalene acetic acid (NAA) and 2 μM 6-benzylaminopurine (BAP). The addition of NAA (4 μM) in the culture medium used for seeds led to an induction rate of 72% in NCs. First, the embryo began to germinate, and afterwards, nodular structures started to form. While NCs formed from seeds is associated with root and shoot meristems, the formation of NCs from leaf explants involves the intercalary meristem. Meristematic cells generate an appropriate response in the induction medium, producing NCs by the proliferation of small cells with meristematic characteristics and large vacuolated cells. These results provide a better understanding of morphogenetic responses in bromeliads and, hence, the opportunity to develop optimized micropropagation protocols. Abbreviations: BAP, 6-benzylaminopurine; 2-iP, N6 (2-isopentyl) adenine; CBB, Coomassie Brilliant Blue; CLSM, confocal laser scanning microscopy; MSB, MS basal medium; NAA, α-Naphthalene acetic acid; NCs, nodular cultures; PAS, Periodic Acid-Schiff; SEM, scanning electron microscopy; TDZ, N-phenyl-N’-1,2,3-thidiazol-5-ylurea; TB-O, Toluidine Blue O; TEM, Transmission electron microscopy.
format Online
Article
Text
id pubmed-6014276
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-60142762018-07-18 Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae) Corredor-Prado, Jenny P. Schmidt, Eder C. Guerra, Miguel P. Bouzon, Zenilda L. Dal Vesco, Lírio L. Pescador, Rosete J Microsc Ultrastruct Original Article Micropropagation via induction, multiplication and development of nodular cultures (NCs) is an efficient regeneration system for Bromeliaceae, a family of endangered monocot plants with ornamental value. Therefore, the present work aimed to induce NCs from seeds and leaf explants of Vriesea in order to characterize the morphological and histochemical aspects of induction and formation of these cultures. Seeds of Vriesea friburgensis var. paludosa were sterilized and inoculated into liquid culture media supplemented with different concentrations and combinations of growth regulators. Leaf explants of Vriesea reitzii were inoculated into medium supplemented with 4 μM α-naphthalene acetic acid (NAA) and 2 μM 6-benzylaminopurine (BAP). The addition of NAA (4 μM) in the culture medium used for seeds led to an induction rate of 72% in NCs. First, the embryo began to germinate, and afterwards, nodular structures started to form. While NCs formed from seeds is associated with root and shoot meristems, the formation of NCs from leaf explants involves the intercalary meristem. Meristematic cells generate an appropriate response in the induction medium, producing NCs by the proliferation of small cells with meristematic characteristics and large vacuolated cells. These results provide a better understanding of morphogenetic responses in bromeliads and, hence, the opportunity to develop optimized micropropagation protocols. Abbreviations: BAP, 6-benzylaminopurine; 2-iP, N6 (2-isopentyl) adenine; CBB, Coomassie Brilliant Blue; CLSM, confocal laser scanning microscopy; MSB, MS basal medium; NAA, α-Naphthalene acetic acid; NCs, nodular cultures; PAS, Periodic Acid-Schiff; SEM, scanning electron microscopy; TDZ, N-phenyl-N’-1,2,3-thidiazol-5-ylurea; TB-O, Toluidine Blue O; TEM, Transmission electron microscopy. Medknow Publications & Media Pvt Ltd 2015 2015-04-16 /pmc/articles/PMC6014276/ /pubmed/30023200 http://dx.doi.org/10.1016/j.jmau.2015.04.001 Text en Copyright: © 2015 Saudi Society of Microscopes http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Corredor-Prado, Jenny P.
Schmidt, Eder C.
Guerra, Miguel P.
Bouzon, Zenilda L.
Dal Vesco, Lírio L.
Pescador, Rosete
Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)
title Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)
title_full Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)
title_fullStr Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)
title_full_unstemmed Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)
title_short Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)
title_sort histodifferentiation and ultrastructure of nodular cultures from seeds of vriesea friburgensis mez var. paludosa (l.b. smith) l.b. smith and leaf explants of vriesea reitzii leme & a. costa (bromeliaceae)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014276/
https://www.ncbi.nlm.nih.gov/pubmed/30023200
http://dx.doi.org/10.1016/j.jmau.2015.04.001
work_keys_str_mv AT corredorpradojennyp histodifferentiationandultrastructureofnodularculturesfromseedsofvrieseafriburgensismezvarpaludosalbsmithlbsmithandleafexplantsofvrieseareitziilemeacostabromeliaceae
AT schmidtederc histodifferentiationandultrastructureofnodularculturesfromseedsofvrieseafriburgensismezvarpaludosalbsmithlbsmithandleafexplantsofvrieseareitziilemeacostabromeliaceae
AT guerramiguelp histodifferentiationandultrastructureofnodularculturesfromseedsofvrieseafriburgensismezvarpaludosalbsmithlbsmithandleafexplantsofvrieseareitziilemeacostabromeliaceae
AT bouzonzenildal histodifferentiationandultrastructureofnodularculturesfromseedsofvrieseafriburgensismezvarpaludosalbsmithlbsmithandleafexplantsofvrieseareitziilemeacostabromeliaceae
AT dalvescoliriol histodifferentiationandultrastructureofnodularculturesfromseedsofvrieseafriburgensismezvarpaludosalbsmithlbsmithandleafexplantsofvrieseareitziilemeacostabromeliaceae
AT pescadorrosete histodifferentiationandultrastructureofnodularculturesfromseedsofvrieseafriburgensismezvarpaludosalbsmithlbsmithandleafexplantsofvrieseareitziilemeacostabromeliaceae