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Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level
Background: Morphine is an opioid analgesic drug often used for pain relief in cancer patients. However, there is growing evidence that morphine may modulate tumor growth, progression and metastasis. Unfortunately, the results obtained by these studies are still contradictory. Methods: In this study...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014364/ https://www.ncbi.nlm.nih.gov/pubmed/27866460 http://dx.doi.org/10.1080/0886022X.2016.1256322 |
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author | Ma, Yabing Ren, Zhongzhong Ma, Shuyong Yan, Wenjun He, Man Wang, Dong Ding, Peiyan |
author_facet | Ma, Yabing Ren, Zhongzhong Ma, Shuyong Yan, Wenjun He, Man Wang, Dong Ding, Peiyan |
author_sort | Ma, Yabing |
collection | PubMed |
description | Background: Morphine is an opioid analgesic drug often used for pain relief in cancer patients. However, there is growing evidence that morphine may modulate tumor growth, progression and metastasis. Unfortunately, the results obtained by these studies are still contradictory. Methods: In this study, we investigated the effect of morphine in human clear cell renal cell carcinoma 786-O, RLC-310 cells and whether morphine affects on tumor growth in human clear cell renal cell carcinoma 786-O, RLC-310 cells. The cell proliferation was determined by MTT assay, cell proliferation, migration and invasion assays. Immunofluorescence staining and Q-PCR was used to determine the Survivin expression. Results: It was shown that morphine enhances proliferation of 786-O, RLC-310 cells, whereas morphine promoted the growth and aggressive phenotype of 786-O and RLC-310 cells in vitro though Survivin-dependent signaling. Conclusions: Our data showed that morphine promotes RCC growth and increases RCC progression via over-expression of Survivin. |
format | Online Article Text |
id | pubmed-6014364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-60143642018-06-28 Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level Ma, Yabing Ren, Zhongzhong Ma, Shuyong Yan, Wenjun He, Man Wang, Dong Ding, Peiyan Ren Fail Laboratory Study Background: Morphine is an opioid analgesic drug often used for pain relief in cancer patients. However, there is growing evidence that morphine may modulate tumor growth, progression and metastasis. Unfortunately, the results obtained by these studies are still contradictory. Methods: In this study, we investigated the effect of morphine in human clear cell renal cell carcinoma 786-O, RLC-310 cells and whether morphine affects on tumor growth in human clear cell renal cell carcinoma 786-O, RLC-310 cells. The cell proliferation was determined by MTT assay, cell proliferation, migration and invasion assays. Immunofluorescence staining and Q-PCR was used to determine the Survivin expression. Results: It was shown that morphine enhances proliferation of 786-O, RLC-310 cells, whereas morphine promoted the growth and aggressive phenotype of 786-O and RLC-310 cells in vitro though Survivin-dependent signaling. Conclusions: Our data showed that morphine promotes RCC growth and increases RCC progression via over-expression of Survivin. Taylor & Francis 2016-11-21 /pmc/articles/PMC6014364/ /pubmed/27866460 http://dx.doi.org/10.1080/0886022X.2016.1256322 Text en © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/Licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Laboratory Study Ma, Yabing Ren, Zhongzhong Ma, Shuyong Yan, Wenjun He, Man Wang, Dong Ding, Peiyan Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
title | Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
title_full | Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
title_fullStr | Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
title_full_unstemmed | Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
title_short | Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
title_sort | morphine enhances renal cell carcinoma aggressiveness through promotes survivin level |
topic | Laboratory Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014364/ https://www.ncbi.nlm.nih.gov/pubmed/27866460 http://dx.doi.org/10.1080/0886022X.2016.1256322 |
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