Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish

The ability to conditionally inactivate genes is instrumental for fine genetic analysis of all biological processes, but is especially important for studies of biological events, such as regeneration, which occur late in ontogenesis or in adult life. We have constructed and tested a fully conditiona...

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Autores principales: Grajevskaja, Viktorija, Camerota, Diana, Bellipanni, Gianfranco, Balciuniene, Jorune, Balciunas, Darius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014646/
https://www.ncbi.nlm.nih.gov/pubmed/29933372
http://dx.doi.org/10.1371/journal.pone.0197293
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author Grajevskaja, Viktorija
Camerota, Diana
Bellipanni, Gianfranco
Balciuniene, Jorune
Balciunas, Darius
author_facet Grajevskaja, Viktorija
Camerota, Diana
Bellipanni, Gianfranco
Balciuniene, Jorune
Balciunas, Darius
author_sort Grajevskaja, Viktorija
collection PubMed
description The ability to conditionally inactivate genes is instrumental for fine genetic analysis of all biological processes, but is especially important for studies of biological events, such as regeneration, which occur late in ontogenesis or in adult life. We have constructed and tested a fully conditional gene trap vector, and used it to inactivate tbx5a in the cardiomyocytes of larval and adult zebrafish. We observe that loss of tbx5a function significantly impairs the ability of zebrafish hearts to regenerate after ventricular resection, indicating that Tbx5a plays an essential role in the transcriptional program of heart regeneration.
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spelling pubmed-60146462018-07-06 Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish Grajevskaja, Viktorija Camerota, Diana Bellipanni, Gianfranco Balciuniene, Jorune Balciunas, Darius PLoS One Research Article The ability to conditionally inactivate genes is instrumental for fine genetic analysis of all biological processes, but is especially important for studies of biological events, such as regeneration, which occur late in ontogenesis or in adult life. We have constructed and tested a fully conditional gene trap vector, and used it to inactivate tbx5a in the cardiomyocytes of larval and adult zebrafish. We observe that loss of tbx5a function significantly impairs the ability of zebrafish hearts to regenerate after ventricular resection, indicating that Tbx5a plays an essential role in the transcriptional program of heart regeneration. Public Library of Science 2018-06-22 /pmc/articles/PMC6014646/ /pubmed/29933372 http://dx.doi.org/10.1371/journal.pone.0197293 Text en © 2018 Grajevskaja et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grajevskaja, Viktorija
Camerota, Diana
Bellipanni, Gianfranco
Balciuniene, Jorune
Balciunas, Darius
Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
title Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
title_full Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
title_fullStr Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
title_full_unstemmed Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
title_short Analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
title_sort analysis of a conditional gene trap reveals that tbx5a is required for heart regeneration in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014646/
https://www.ncbi.nlm.nih.gov/pubmed/29933372
http://dx.doi.org/10.1371/journal.pone.0197293
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