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Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system
BACKGROUND: Melanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America. OBJECTIVE: The aim of this study was to evaluate the effects o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014723/ https://www.ncbi.nlm.nih.gov/pubmed/29947713 http://dx.doi.org/10.1590/0074-02760180120 |
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author | Pinto, Lysianne Granja, Luiz Fernando Zmetek de Almeida, Mariana Amorim Alviano, Daniela Sales da Silva, Maria Helena Ejzemberg, Regina Rozental, Sonia Alviano, Celuta Sales |
author_facet | Pinto, Lysianne Granja, Luiz Fernando Zmetek de Almeida, Mariana Amorim Alviano, Daniela Sales da Silva, Maria Helena Ejzemberg, Regina Rozental, Sonia Alviano, Celuta Sales |
author_sort | Pinto, Lysianne |
collection | PubMed |
description | BACKGROUND: Melanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America. OBJECTIVE: The aim of this study was to evaluate the effects of acid-basic extracted F. pedrosoi melanin particles and fungal cell ghosts obtained by Novozym 234 treatment on their ability to activate the human complement system. METHODS: The ability of melanin particles and fungal cell ghosts to activate the human complement system was evaluated by complement consumption, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). FINDINGS: Unsensitised melanin particles and melanin ghosts presented complement consumption of 82.67 ± 2.08% and 96.04 ± 1.13%, respectively. Immunofluorescence assays revealed intense deposition of the C3 and C4 fragments on the surface of melanin particles and ghosts extracted from F. pedrosoi. Deposition of the C3, C4, and C5 fragments onto melanin samples and zymosan was confirmed by ELISA. Deposition of small amounts of C1q and C9 onto melanin samples and zymosan was detected by ELISA. CONCLUSION: Fonsecaea pedrosoi melanin particles and fungal cell ghosts activated the complement system mainly through an alternative pathway. |
format | Online Article Text |
id | pubmed-6014723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-60147232018-06-26 Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system Pinto, Lysianne Granja, Luiz Fernando Zmetek de Almeida, Mariana Amorim Alviano, Daniela Sales da Silva, Maria Helena Ejzemberg, Regina Rozental, Sonia Alviano, Celuta Sales Mem Inst Oswaldo Cruz Original Article BACKGROUND: Melanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America. OBJECTIVE: The aim of this study was to evaluate the effects of acid-basic extracted F. pedrosoi melanin particles and fungal cell ghosts obtained by Novozym 234 treatment on their ability to activate the human complement system. METHODS: The ability of melanin particles and fungal cell ghosts to activate the human complement system was evaluated by complement consumption, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). FINDINGS: Unsensitised melanin particles and melanin ghosts presented complement consumption of 82.67 ± 2.08% and 96.04 ± 1.13%, respectively. Immunofluorescence assays revealed intense deposition of the C3 and C4 fragments on the surface of melanin particles and ghosts extracted from F. pedrosoi. Deposition of the C3, C4, and C5 fragments onto melanin samples and zymosan was confirmed by ELISA. Deposition of small amounts of C1q and C9 onto melanin samples and zymosan was detected by ELISA. CONCLUSION: Fonsecaea pedrosoi melanin particles and fungal cell ghosts activated the complement system mainly through an alternative pathway. Instituto Oswaldo Cruz, Ministério da Saúde 2018-06-25 /pmc/articles/PMC6014723/ /pubmed/29947713 http://dx.doi.org/10.1590/0074-02760180120 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Pinto, Lysianne Granja, Luiz Fernando Zmetek de Almeida, Mariana Amorim Alviano, Daniela Sales da Silva, Maria Helena Ejzemberg, Regina Rozental, Sonia Alviano, Celuta Sales Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system |
title | Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system |
title_full | Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system |
title_fullStr | Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system |
title_full_unstemmed | Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system |
title_short | Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system |
title_sort | melanin particles isolated from the fungus fonsecaea pedrosoi activates the human complement system |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014723/ https://www.ncbi.nlm.nih.gov/pubmed/29947713 http://dx.doi.org/10.1590/0074-02760180120 |
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