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Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014725/ https://www.ncbi.nlm.nih.gov/pubmed/29932052 http://dx.doi.org/10.7554/eLife.34700 |
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author | Pfeiffer, Thomas Poll, Stefanie Bancelin, Stephane Angibaud, Julie Inavalli, VVG Krishna Keppler, Kevin Mittag, Manuel Fuhrmann, Martin Nägerl, U Valentin |
author_facet | Pfeiffer, Thomas Poll, Stefanie Bancelin, Stephane Angibaud, Julie Inavalli, VVG Krishna Keppler, Kevin Mittag, Manuel Fuhrmann, Martin Nägerl, U Valentin |
author_sort | Pfeiffer, Thomas |
collection | PubMed |
description | Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments. |
format | Online Article Text |
id | pubmed-6014725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60147252018-06-25 Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo Pfeiffer, Thomas Poll, Stefanie Bancelin, Stephane Angibaud, Julie Inavalli, VVG Krishna Keppler, Kevin Mittag, Manuel Fuhrmann, Martin Nägerl, U Valentin eLife Neuroscience Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments. eLife Sciences Publications, Ltd 2018-06-22 /pmc/articles/PMC6014725/ /pubmed/29932052 http://dx.doi.org/10.7554/eLife.34700 Text en © 2018, Pfeiffer et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Pfeiffer, Thomas Poll, Stefanie Bancelin, Stephane Angibaud, Julie Inavalli, VVG Krishna Keppler, Kevin Mittag, Manuel Fuhrmann, Martin Nägerl, U Valentin Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
title | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
title_full | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
title_fullStr | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
title_full_unstemmed | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
title_short | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
title_sort | chronic 2p-sted imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014725/ https://www.ncbi.nlm.nih.gov/pubmed/29932052 http://dx.doi.org/10.7554/eLife.34700 |
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