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Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo

Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensi...

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Autores principales: Pfeiffer, Thomas, Poll, Stefanie, Bancelin, Stephane, Angibaud, Julie, Inavalli, VVG Krishna, Keppler, Kevin, Mittag, Manuel, Fuhrmann, Martin, Nägerl, U Valentin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014725/
https://www.ncbi.nlm.nih.gov/pubmed/29932052
http://dx.doi.org/10.7554/eLife.34700
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author Pfeiffer, Thomas
Poll, Stefanie
Bancelin, Stephane
Angibaud, Julie
Inavalli, VVG Krishna
Keppler, Kevin
Mittag, Manuel
Fuhrmann, Martin
Nägerl, U Valentin
author_facet Pfeiffer, Thomas
Poll, Stefanie
Bancelin, Stephane
Angibaud, Julie
Inavalli, VVG Krishna
Keppler, Kevin
Mittag, Manuel
Fuhrmann, Martin
Nägerl, U Valentin
author_sort Pfeiffer, Thomas
collection PubMed
description Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments.
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spelling pubmed-60147252018-06-25 Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo Pfeiffer, Thomas Poll, Stefanie Bancelin, Stephane Angibaud, Julie Inavalli, VVG Krishna Keppler, Kevin Mittag, Manuel Fuhrmann, Martin Nägerl, U Valentin eLife Neuroscience Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments. eLife Sciences Publications, Ltd 2018-06-22 /pmc/articles/PMC6014725/ /pubmed/29932052 http://dx.doi.org/10.7554/eLife.34700 Text en © 2018, Pfeiffer et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Pfeiffer, Thomas
Poll, Stefanie
Bancelin, Stephane
Angibaud, Julie
Inavalli, VVG Krishna
Keppler, Kevin
Mittag, Manuel
Fuhrmann, Martin
Nägerl, U Valentin
Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
title Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
title_full Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
title_fullStr Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
title_full_unstemmed Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
title_short Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
title_sort chronic 2p-sted imaging reveals high turnover of dendritic spines in the hippocampus in vivo
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014725/
https://www.ncbi.nlm.nih.gov/pubmed/29932052
http://dx.doi.org/10.7554/eLife.34700
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