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Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism

This work was designed to separate and purify the saponin from Thelenota ananas with the highest anti-cholesterol ability using multiple chromatography and mass spectrometry analyses, and to systematically investigate the effect of the Thelenota ananas saponin on cholesterol metabolism in oxidized l...

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Autores principales: Han, Qi-an, Li, Kaifeng, Dong, Xiuping, Luo, Yongkang, Zhu, Beiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014995/
https://www.ncbi.nlm.nih.gov/pubmed/29934523
http://dx.doi.org/10.1038/s41598-018-27932-x
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author Han, Qi-an
Li, Kaifeng
Dong, Xiuping
Luo, Yongkang
Zhu, Beiwei
author_facet Han, Qi-an
Li, Kaifeng
Dong, Xiuping
Luo, Yongkang
Zhu, Beiwei
author_sort Han, Qi-an
collection PubMed
description This work was designed to separate and purify the saponin from Thelenota ananas with the highest anti-cholesterol ability using multiple chromatography and mass spectrometry analyses, and to systematically investigate the effect of the Thelenota ananas saponin on cholesterol metabolism in oxidized low-density lipoprotein (ox-LDL) induced macrophage foam cells. Desulfated holothurin A (desHA), which was finally identified as the targeted saponin with the highest activity in decreasing low-density lipoprotein-cholesterol (LDL-C), markedly inhibited the formation of foam cells derived from macrophages based on Oil Red O staining. In addition, desHA significantly blocked the synthesis of fatty acid synthetase while promoted intracellular cholesterol efflux. Furthermore, desHA inhibited the effects of ox-LDL on macrophage mRNA expression, which enhanced the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoAR) and suppressed the expression of SR-BI, ABCA1 and ABCG1, which further increased the levels of extracellular cholesterol and triglyceride. Blocking AKT and AMPK pathway and LXR synthesis revealed that desHA also regulated the contents of HMG-CoAR and eNOS via LXR/AKT/AMPK pathway. Thus, desHA played an essential role in cholesterol efflux and synthesis, which indicated desHA and Thelenota ananas are valuable resources to exploit new functional food and nutraceuticals.
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spelling pubmed-60149952018-07-06 Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism Han, Qi-an Li, Kaifeng Dong, Xiuping Luo, Yongkang Zhu, Beiwei Sci Rep Article This work was designed to separate and purify the saponin from Thelenota ananas with the highest anti-cholesterol ability using multiple chromatography and mass spectrometry analyses, and to systematically investigate the effect of the Thelenota ananas saponin on cholesterol metabolism in oxidized low-density lipoprotein (ox-LDL) induced macrophage foam cells. Desulfated holothurin A (desHA), which was finally identified as the targeted saponin with the highest activity in decreasing low-density lipoprotein-cholesterol (LDL-C), markedly inhibited the formation of foam cells derived from macrophages based on Oil Red O staining. In addition, desHA significantly blocked the synthesis of fatty acid synthetase while promoted intracellular cholesterol efflux. Furthermore, desHA inhibited the effects of ox-LDL on macrophage mRNA expression, which enhanced the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoAR) and suppressed the expression of SR-BI, ABCA1 and ABCG1, which further increased the levels of extracellular cholesterol and triglyceride. Blocking AKT and AMPK pathway and LXR synthesis revealed that desHA also regulated the contents of HMG-CoAR and eNOS via LXR/AKT/AMPK pathway. Thus, desHA played an essential role in cholesterol efflux and synthesis, which indicated desHA and Thelenota ananas are valuable resources to exploit new functional food and nutraceuticals. Nature Publishing Group UK 2018-06-22 /pmc/articles/PMC6014995/ /pubmed/29934523 http://dx.doi.org/10.1038/s41598-018-27932-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Han, Qi-an
Li, Kaifeng
Dong, Xiuping
Luo, Yongkang
Zhu, Beiwei
Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism
title Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism
title_full Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism
title_fullStr Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism
title_full_unstemmed Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism
title_short Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism
title_sort function of thelenota ananas saponin desulfated holothurin a in modulating cholesterol metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014995/
https://www.ncbi.nlm.nih.gov/pubmed/29934523
http://dx.doi.org/10.1038/s41598-018-27932-x
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