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Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species
Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and phenotypic changes induced by salt treatments (10–50 mM NaCl and Na(2)SO(4) mixtures) were analy...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015060/ https://www.ncbi.nlm.nih.gov/pubmed/29934583 http://dx.doi.org/10.1038/s41598-018-27935-8 |
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author | Long, Ruicai Gao, Yanli Sun, Hao Zhang, Tiejun Li, Xiao Li, Mingna Sun, Yan Kang, Junmei Wang, Zhen Ding, Wang Yang, Qingchuan |
author_facet | Long, Ruicai Gao, Yanli Sun, Hao Zhang, Tiejun Li, Xiao Li, Mingna Sun, Yan Kang, Junmei Wang, Zhen Ding, Wang Yang, Qingchuan |
author_sort | Long, Ruicai |
collection | PubMed |
description | Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and phenotypic changes induced by salt treatments (10–50 mM NaCl and Na(2)SO(4) mixtures) were analysed for Zhongmu-3 (Medicago sativa) and R108 (Medicago truncatula) seedlings. Our observations indicated that Zhongmu-3 was more salt-tolerant than R108. To characterize the protein expression profiles of these two Medicago species in response to salt stress, an iTRAQ-based quantitative proteomic analysis was applied to examine salt-responsive proteins. We identified 254 differentially changed salt-responsive proteins. Compared with control levels, the abundance of 121 proteins increased and 44 proteins decreased in salt-treated Zhongmu-3 seedlings, while 119 proteins increased and 18 proteins decreased in R108 seedlings. Moreover, 48 differentially changed proteins were common to Zhongmu-3 and R108 seedlings. A subsequent functional annotation indicated these proteins influenced diverse processes, such as catalytic activity, binding, and antioxidant activity. Furthermore, the corresponding transcript levels of 15 differentially changed proteins were quantified by qRT-PCR. The data presented herein provide new insights into salt-responsive proteins, with potential implications for enhancing the salt tolerance of Medicago species. |
format | Online Article Text |
id | pubmed-6015060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60150602018-07-06 Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species Long, Ruicai Gao, Yanli Sun, Hao Zhang, Tiejun Li, Xiao Li, Mingna Sun, Yan Kang, Junmei Wang, Zhen Ding, Wang Yang, Qingchuan Sci Rep Article Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and phenotypic changes induced by salt treatments (10–50 mM NaCl and Na(2)SO(4) mixtures) were analysed for Zhongmu-3 (Medicago sativa) and R108 (Medicago truncatula) seedlings. Our observations indicated that Zhongmu-3 was more salt-tolerant than R108. To characterize the protein expression profiles of these two Medicago species in response to salt stress, an iTRAQ-based quantitative proteomic analysis was applied to examine salt-responsive proteins. We identified 254 differentially changed salt-responsive proteins. Compared with control levels, the abundance of 121 proteins increased and 44 proteins decreased in salt-treated Zhongmu-3 seedlings, while 119 proteins increased and 18 proteins decreased in R108 seedlings. Moreover, 48 differentially changed proteins were common to Zhongmu-3 and R108 seedlings. A subsequent functional annotation indicated these proteins influenced diverse processes, such as catalytic activity, binding, and antioxidant activity. Furthermore, the corresponding transcript levels of 15 differentially changed proteins were quantified by qRT-PCR. The data presented herein provide new insights into salt-responsive proteins, with potential implications for enhancing the salt tolerance of Medicago species. Nature Publishing Group UK 2018-06-22 /pmc/articles/PMC6015060/ /pubmed/29934583 http://dx.doi.org/10.1038/s41598-018-27935-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Long, Ruicai Gao, Yanli Sun, Hao Zhang, Tiejun Li, Xiao Li, Mingna Sun, Yan Kang, Junmei Wang, Zhen Ding, Wang Yang, Qingchuan Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species |
title | Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species |
title_full | Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species |
title_fullStr | Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species |
title_full_unstemmed | Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species |
title_short | Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species |
title_sort | quantitative proteomic analysis using itraq to identify salt-responsive proteins during the germination stage of two medicago species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015060/ https://www.ncbi.nlm.nih.gov/pubmed/29934583 http://dx.doi.org/10.1038/s41598-018-27935-8 |
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