Cargando…

Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)

Although nitrate is known to inhibit nodulation and N(2) fixation in symbiotic legumes, little is known about its effect on the uptake and accumulation of trace elements such as Fe, Zn, Mn and Cu. The aim of this study was to evaluate the effect of 5 mM NO(3) (−) supply, either with or without rhizo...

Descripción completa

Detalles Bibliográficos
Autores principales: Mbah, Glory Chinonye, Dakora, Felix Dapare
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015606/
https://www.ncbi.nlm.nih.gov/pubmed/29997415
http://dx.doi.org/10.1007/s13199-017-0531-2
_version_ 1783334434775236608
author Mbah, Glory Chinonye
Dakora, Felix Dapare
author_facet Mbah, Glory Chinonye
Dakora, Felix Dapare
author_sort Mbah, Glory Chinonye
collection PubMed
description Although nitrate is known to inhibit nodulation and N(2) fixation in symbiotic legumes, little is known about its effect on the uptake and accumulation of trace elements such as Fe, Zn, Mn and Cu. The aim of this study was to evaluate the effect of 5 mM NO(3) (−) supply, either with or without rhizobial inoculation, on nodulation, nodule functioning and micronutrient levels in the shoots of soybean (Glycine max L.Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harm). The results showed reduction in plant growth, nodule formation and nodule dry matter by the supply of 5 mM NO(3) (−) to inoculated seedlings of all three species. Nitrate inhibition respectively caused 1.2, 1.4, and 1.5-fold decrease in nodule number per plant in Bambara groundnut, soybean and Kersting’s bean, which resulted in 2.3, 3.3 and 4.5-fold reduction in nodule dry weight of the test species (in that order). The application of 5 mM NO(3) (−) to soybean plants also resulted in 2.5, 4.0 and 5.4-fold decrease in shoot accumulation of Fe, Zn and Mn, respectively, when compared to the purely symbiotic control plants. Furthermore, we observed 1.3, 1.8 and 1.3-fold decreases in the concentration of Zn, Mn and Cu in shoots of inoculated Bambara groundnut with NO(3) (−) supply, levels lower than those found in soybean. With Kersting’s groundnut, shoot concentration of Fe, Zn and Cu were higher with the application of 5 mM NO(3) (−) to inoculated plants when compared to the purely symbiotic treatment, which was opposite to soybean. But pure NO(3) (−)feeding of this species respectively resulted in 2.0, 1.4 and 1.3-fold decreases in Fe, Zn and Cu relative to inoculated NO(3) (−)-fed plants. Clearly, NO(3) (−) supply to landraces/genotypes of the three legume species did not only inhibit nodule formation and functioning, it also reduced shoot micronutrient levels in soybean and Bambara groundnut, but not Kersting’s bean.
format Online
Article
Text
id pubmed-6015606
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-60156062018-07-09 Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.) Mbah, Glory Chinonye Dakora, Felix Dapare Symbiosis Article Although nitrate is known to inhibit nodulation and N(2) fixation in symbiotic legumes, little is known about its effect on the uptake and accumulation of trace elements such as Fe, Zn, Mn and Cu. The aim of this study was to evaluate the effect of 5 mM NO(3) (−) supply, either with or without rhizobial inoculation, on nodulation, nodule functioning and micronutrient levels in the shoots of soybean (Glycine max L.Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harm). The results showed reduction in plant growth, nodule formation and nodule dry matter by the supply of 5 mM NO(3) (−) to inoculated seedlings of all three species. Nitrate inhibition respectively caused 1.2, 1.4, and 1.5-fold decrease in nodule number per plant in Bambara groundnut, soybean and Kersting’s bean, which resulted in 2.3, 3.3 and 4.5-fold reduction in nodule dry weight of the test species (in that order). The application of 5 mM NO(3) (−) to soybean plants also resulted in 2.5, 4.0 and 5.4-fold decrease in shoot accumulation of Fe, Zn and Mn, respectively, when compared to the purely symbiotic control plants. Furthermore, we observed 1.3, 1.8 and 1.3-fold decreases in the concentration of Zn, Mn and Cu in shoots of inoculated Bambara groundnut with NO(3) (−) supply, levels lower than those found in soybean. With Kersting’s groundnut, shoot concentration of Fe, Zn and Cu were higher with the application of 5 mM NO(3) (−) to inoculated plants when compared to the purely symbiotic treatment, which was opposite to soybean. But pure NO(3) (−)feeding of this species respectively resulted in 2.0, 1.4 and 1.3-fold decreases in Fe, Zn and Cu relative to inoculated NO(3) (−)-fed plants. Clearly, NO(3) (−) supply to landraces/genotypes of the three legume species did not only inhibit nodule formation and functioning, it also reduced shoot micronutrient levels in soybean and Bambara groundnut, but not Kersting’s bean. Springer Netherlands 2017-12-15 2018 /pmc/articles/PMC6015606/ /pubmed/29997415 http://dx.doi.org/10.1007/s13199-017-0531-2 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Mbah, Glory Chinonye
Dakora, Felix Dapare
Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)
title Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)
title_full Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)
title_fullStr Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)
title_full_unstemmed Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)
title_short Nitrate inhibition of N(2) fixation and its effect on micronutrient accumulation in shoots of soybean (Glycine max L. Merr.), Bambara groundnut (Vigna subterranea L. Vedc) and Kersting’s groundnut (Macrotyloma geocarpum Harms.)
title_sort nitrate inhibition of n(2) fixation and its effect on micronutrient accumulation in shoots of soybean (glycine max l. merr.), bambara groundnut (vigna subterranea l. vedc) and kersting’s groundnut (macrotyloma geocarpum harms.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015606/
https://www.ncbi.nlm.nih.gov/pubmed/29997415
http://dx.doi.org/10.1007/s13199-017-0531-2
work_keys_str_mv AT mbahglorychinonye nitrateinhibitionofn2fixationanditseffectonmicronutrientaccumulationinshootsofsoybeanglycinemaxlmerrbambaragroundnutvignasubterranealvedcandkerstingsgroundnutmacrotylomageocarpumharms
AT dakorafelixdapare nitrateinhibitionofn2fixationanditseffectonmicronutrientaccumulationinshootsofsoybeanglycinemaxlmerrbambaragroundnutvignasubterranealvedcandkerstingsgroundnutmacrotylomageocarpumharms