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Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo

There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to test in vivo antiplasmodial activity of Croton macrostachyus H. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with er...

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Autores principales: Obey, Jackie K., Ngeiywa, Moses M., Kiprono, Paul, Omar, Sabah, von Wright, Atte, Kauhanen, Jussi, Tikkanen-Kaukanen, Carina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015669/
https://www.ncbi.nlm.nih.gov/pubmed/29984002
http://dx.doi.org/10.1155/2018/2393854
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author Obey, Jackie K.
Ngeiywa, Moses M.
Kiprono, Paul
Omar, Sabah
von Wright, Atte
Kauhanen, Jussi
Tikkanen-Kaukanen, Carina
author_facet Obey, Jackie K.
Ngeiywa, Moses M.
Kiprono, Paul
Omar, Sabah
von Wright, Atte
Kauhanen, Jussi
Tikkanen-Kaukanen, Carina
author_sort Obey, Jackie K.
collection PubMed
description There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to test in vivo antiplasmodial activity of Croton macrostachyus H. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized with Plasmodium berghei (ANKA). Different doses (500, 250, and 100 mg/kg) of C. macrostachyus ethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters' 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control (p < 0.001): for the ethyl acetate extract in the range of 58–82%, for the methanol extract in the range of 27–68%, for the aqueous extract in the range of 24–72%, and for the isobutanol extract in the range of 61–80%. Chemoprotective effect was significant (p < 0.001) and the suppression caused by the ethyl acetate extract was between 74 and 100%, by the methanol extract between 57 and 83%, and by the isobutanol extract between 86–92%. The study showed that it is possible to inhibit the growth of the parasites by various stem bark extracts of C. macrostachyus in Balb/c mice supporting the folkloric use of the plant against malaria.
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spelling pubmed-60156692018-07-08 Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo Obey, Jackie K. Ngeiywa, Moses M. Kiprono, Paul Omar, Sabah von Wright, Atte Kauhanen, Jussi Tikkanen-Kaukanen, Carina J Pathog Research Article There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to test in vivo antiplasmodial activity of Croton macrostachyus H. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized with Plasmodium berghei (ANKA). Different doses (500, 250, and 100 mg/kg) of C. macrostachyus ethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters' 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control (p < 0.001): for the ethyl acetate extract in the range of 58–82%, for the methanol extract in the range of 27–68%, for the aqueous extract in the range of 24–72%, and for the isobutanol extract in the range of 61–80%. Chemoprotective effect was significant (p < 0.001) and the suppression caused by the ethyl acetate extract was between 74 and 100%, by the methanol extract between 57 and 83%, and by the isobutanol extract between 86–92%. The study showed that it is possible to inhibit the growth of the parasites by various stem bark extracts of C. macrostachyus in Balb/c mice supporting the folkloric use of the plant against malaria. Hindawi 2018-06-10 /pmc/articles/PMC6015669/ /pubmed/29984002 http://dx.doi.org/10.1155/2018/2393854 Text en Copyright © 2018 Jackie K. Obey et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Obey, Jackie K.
Ngeiywa, Moses M.
Kiprono, Paul
Omar, Sabah
von Wright, Atte
Kauhanen, Jussi
Tikkanen-Kaukanen, Carina
Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo
title Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo
title_full Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo
title_fullStr Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo
title_full_unstemmed Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo
title_short Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo
title_sort antimalarial activity of croton macrostachyus stem bark extracts against plasmodium berghei in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015669/
https://www.ncbi.nlm.nih.gov/pubmed/29984002
http://dx.doi.org/10.1155/2018/2393854
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