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Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides

The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been t...

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Autores principales: Aguilar-Díaz, H., Esquivel-Velázquez, M., Quiroz-Castañeda, R. E., Miranda-Miranda, E., Conde-Baeye, R. J. P., Cobaxín-Cárdenas, M., Ostoa-Saloma, P., Cossío-Bayúgar, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016230/
https://www.ncbi.nlm.nih.gov/pubmed/29992168
http://dx.doi.org/10.1155/2018/9451547
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author Aguilar-Díaz, H.
Esquivel-Velázquez, M.
Quiroz-Castañeda, R. E.
Miranda-Miranda, E.
Conde-Baeye, R. J. P.
Cobaxín-Cárdenas, M.
Ostoa-Saloma, P.
Cossío-Bayúgar, R.
author_facet Aguilar-Díaz, H.
Esquivel-Velázquez, M.
Quiroz-Castañeda, R. E.
Miranda-Miranda, E.
Conde-Baeye, R. J. P.
Cobaxín-Cárdenas, M.
Ostoa-Saloma, P.
Cossío-Bayúgar, R.
author_sort Aguilar-Díaz, H.
collection PubMed
description The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been the use of ixodicides, resulting in chemically resistant tick populations. It is necessary to understand the mechanisms that result in resistance so as to create new strategies increasing the lifespan of ixodicides or finding alternative targets to produce new acaricides. In this paper, in order to obtain an insight into the mechanisms that govern ixodicides resistance, we will compare the hemolymph proteome of two tick R. microplus strains, one susceptible (MJ) and one resistant (SA) to ixodicides, using HPLC and 2D electrophoresis. Significant differences were found in protein content between strains using HPLC. 2D electrophoresis revealed that 68 hemolymph protein spots were common between strains; however, 26 spots were unique to the susceptible strain MJ and 5 to the resistant strain SA. The most distinctive protein spots on the preparative gels were selected for further analyses. Nine protein spots were identified by mass fingerprinting, 
revealing proteins that may have a role in the ixodicides resistance or susceptibility. In this paper, we present the tick hemolymph proteome revealing a set of proteins which suggest a possible role in tick detoxification.
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spelling pubmed-60162302018-07-10 Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides Aguilar-Díaz, H. Esquivel-Velázquez, M. Quiroz-Castañeda, R. E. Miranda-Miranda, E. Conde-Baeye, R. J. P. Cobaxín-Cárdenas, M. Ostoa-Saloma, P. Cossío-Bayúgar, R. Biomed Res Int Research Article The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been the use of ixodicides, resulting in chemically resistant tick populations. It is necessary to understand the mechanisms that result in resistance so as to create new strategies increasing the lifespan of ixodicides or finding alternative targets to produce new acaricides. In this paper, in order to obtain an insight into the mechanisms that govern ixodicides resistance, we will compare the hemolymph proteome of two tick R. microplus strains, one susceptible (MJ) and one resistant (SA) to ixodicides, using HPLC and 2D electrophoresis. Significant differences were found in protein content between strains using HPLC. 2D electrophoresis revealed that 68 hemolymph protein spots were common between strains; however, 26 spots were unique to the susceptible strain MJ and 5 to the resistant strain SA. The most distinctive protein spots on the preparative gels were selected for further analyses. Nine protein spots were identified by mass fingerprinting, 
revealing proteins that may have a role in the ixodicides resistance or susceptibility. In this paper, we present the tick hemolymph proteome revealing a set of proteins which suggest a possible role in tick detoxification. Hindawi 2018-06-11 /pmc/articles/PMC6016230/ /pubmed/29992168 http://dx.doi.org/10.1155/2018/9451547 Text en Copyright © 2018 H. Aguilar-Díaz et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Aguilar-Díaz, H.
Esquivel-Velázquez, M.
Quiroz-Castañeda, R. E.
Miranda-Miranda, E.
Conde-Baeye, R. J. P.
Cobaxín-Cárdenas, M.
Ostoa-Saloma, P.
Cossío-Bayúgar, R.
Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
title Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
title_full Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
title_fullStr Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
title_full_unstemmed Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
title_short Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
title_sort comparative hemolymph proteomic and enzymatic analyses of two strains of rhipicephalus (boophilus) microplus ticks resistant and susceptible to ixodicides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016230/
https://www.ncbi.nlm.nih.gov/pubmed/29992168
http://dx.doi.org/10.1155/2018/9451547
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