Cargando…

Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016

BACKGROUND: Drug resistance is a leading concern in control of TB. Resistance against rifampin as one of the most important drugs in the treatment of Mycobacterium tuberculosis is caused by mutations in the 81-base pair region of the rpoB gene encoding the β-subunit of RNA polymerase. This study aim...

Descripción completa

Detalles Bibliográficos
Autores principales: Tavanaee sani, Ashraf, Ashna, Habib, Kaffash, Afsaneh, Khaledi, Azad, Ghazvini, Kiarash
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Publications Office of Jimma University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016358/
https://www.ncbi.nlm.nih.gov/pubmed/29983529
http://dx.doi.org/10.4314/ejhs.v28i3.7
_version_ 1783334559872450560
author Tavanaee sani, Ashraf
Ashna, Habib
Kaffash, Afsaneh
Khaledi, Azad
Ghazvini, Kiarash
author_facet Tavanaee sani, Ashraf
Ashna, Habib
Kaffash, Afsaneh
Khaledi, Azad
Ghazvini, Kiarash
author_sort Tavanaee sani, Ashraf
collection PubMed
description BACKGROUND: Drug resistance is a leading concern in control of TB. Resistance against rifampin as one of the most important drugs in the treatment of Mycobacterium tuberculosis is caused by mutations in the 81-base pair region of the rpoB gene encoding the β-subunit of RNA polymerase. This study aimed to characterize the mutations in the rpoB gene associated with rifampin resistance among M. tuberculosis. METHODS: This study was conducted on referred samples of patients who did not respond to anti-TB treatment, in Tuberculosis Regional Reference Laboratory at Shariati Hospital. Drug susceptibility of M. tuberculosis isolates was surveyed using a proportional method on LJ medium. The isolates with resistant to rifampin were reconfirmed and then the rpoB gene was amplified and sequenced. RESULTS: Among 27 resistant cases, 8, 11 and 8 people were from Iran, Afghanistan, and Turkmenistan, respectively. In 26 out of 27 isolates, rpoB gene mutations were observed. The most prevalent mutations belonged to the codon 53. The most prevalent mutations belonged to the TCG (Ser) 531TTG (leu) with prevalence 51.8% (n=14), and GAC (Asp)516TAC (Tyr), CAC (His) 526GAC (Asp) and CAC (His) 526TAC(Tyr) mutations with prevalence 14.8%(n=4). Twenty-three isolates had just one mutation. CONCLUSION: The use of rpoB gene sequencing led to the lack of the need for growth of the organism in the culture medium, the direct use of clinical samples, reduction of biological risks and a detection about 96.3% of MDR TB cases lowering the cost of the treatment.
format Online
Article
Text
id pubmed-6016358
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Research and Publications Office of Jimma University
record_format MEDLINE/PubMed
spelling pubmed-60163582018-07-06 Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016 Tavanaee sani, Ashraf Ashna, Habib Kaffash, Afsaneh Khaledi, Azad Ghazvini, Kiarash Ethiop J Health Sci Original Article BACKGROUND: Drug resistance is a leading concern in control of TB. Resistance against rifampin as one of the most important drugs in the treatment of Mycobacterium tuberculosis is caused by mutations in the 81-base pair region of the rpoB gene encoding the β-subunit of RNA polymerase. This study aimed to characterize the mutations in the rpoB gene associated with rifampin resistance among M. tuberculosis. METHODS: This study was conducted on referred samples of patients who did not respond to anti-TB treatment, in Tuberculosis Regional Reference Laboratory at Shariati Hospital. Drug susceptibility of M. tuberculosis isolates was surveyed using a proportional method on LJ medium. The isolates with resistant to rifampin were reconfirmed and then the rpoB gene was amplified and sequenced. RESULTS: Among 27 resistant cases, 8, 11 and 8 people were from Iran, Afghanistan, and Turkmenistan, respectively. In 26 out of 27 isolates, rpoB gene mutations were observed. The most prevalent mutations belonged to the codon 53. The most prevalent mutations belonged to the TCG (Ser) 531TTG (leu) with prevalence 51.8% (n=14), and GAC (Asp)516TAC (Tyr), CAC (His) 526GAC (Asp) and CAC (His) 526TAC(Tyr) mutations with prevalence 14.8%(n=4). Twenty-three isolates had just one mutation. CONCLUSION: The use of rpoB gene sequencing led to the lack of the need for growth of the organism in the culture medium, the direct use of clinical samples, reduction of biological risks and a detection about 96.3% of MDR TB cases lowering the cost of the treatment. Research and Publications Office of Jimma University 2018-05 /pmc/articles/PMC6016358/ /pubmed/29983529 http://dx.doi.org/10.4314/ejhs.v28i3.7 Text en © 2018 Tavanaee, A. et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Tavanaee sani, Ashraf
Ashna, Habib
Kaffash, Afsaneh
Khaledi, Azad
Ghazvini, Kiarash
Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016
title Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016
title_full Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016
title_fullStr Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016
title_full_unstemmed Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016
title_short Mutations of rpob Gene Associated with Rifampin Resistance among Mycobacterium Tuberculosis Isolated in Tuberculosis Regional Reference Laboratory in Northeast of Iran during 2015–2016
title_sort mutations of rpob gene associated with rifampin resistance among mycobacterium tuberculosis isolated in tuberculosis regional reference laboratory in northeast of iran during 2015–2016
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016358/
https://www.ncbi.nlm.nih.gov/pubmed/29983529
http://dx.doi.org/10.4314/ejhs.v28i3.7
work_keys_str_mv AT tavanaeesaniashraf mutationsofrpobgeneassociatedwithrifampinresistanceamongmycobacteriumtuberculosisisolatedintuberculosisregionalreferencelaboratoryinnortheastofiranduring20152016
AT ashnahabib mutationsofrpobgeneassociatedwithrifampinresistanceamongmycobacteriumtuberculosisisolatedintuberculosisregionalreferencelaboratoryinnortheastofiranduring20152016
AT kaffashafsaneh mutationsofrpobgeneassociatedwithrifampinresistanceamongmycobacteriumtuberculosisisolatedintuberculosisregionalreferencelaboratoryinnortheastofiranduring20152016
AT khalediazad mutationsofrpobgeneassociatedwithrifampinresistanceamongmycobacteriumtuberculosisisolatedintuberculosisregionalreferencelaboratoryinnortheastofiranduring20152016
AT ghazvinikiarash mutationsofrpobgeneassociatedwithrifampinresistanceamongmycobacteriumtuberculosisisolatedintuberculosisregionalreferencelaboratoryinnortheastofiranduring20152016